Water soluble chitin pH probe and producing method thereof

A water-soluble chitin and probe technology, applied in the field of chemistry, can solve problems such as less research, achieve the effects of process safety, broaden the scope of application, and avoid damage to the internal structure

Inactive Publication Date: 2007-06-06
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are commercial products for the probes used in the cell fluid, but there are few studies on the probes used in acidic organelles, but if the probes can be extended to acidic environments, it will be of great significance to the diagnosis of diseases in cells

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Example 1: To 100 mL of 10 mg / mL water-soluble chitin solution with a deacetylation degree of 48%, 100 mL of 0.023 mg / mL 9-anthracene methanol solution was added, the mixture was stirred for 8 hours, and then 1 mg of cyano was added Sodium borohydride, continue to stir for 12h, the reaction solution is rotary evaporated, and the concentrated solution is soaked in deionized water for 3-4 days with a 0.8-10,000 dialysis bag, and then the product is extracted with ether until no 9- anthralde. The solution was then lyophilized. A water-soluble chitin pH probe with a substitution degree of 0.2% was obtained.

Embodiment 2

[0011] Example 2: Add 100 mL of 0.057 mg / mL 9-anthracenaldehyde methanol solution to 100 mL of 10 mg / mL water-soluble chitin solution with a deacetylation degree of 53%, stir the mixture for 8 hours, and then add 2.0 mg of cyanide Base sodium borohydride, continue to stir for 12h, the reaction solution is rotary evaporated, and the concentrated solution is soaked in deionized water for 3-4 days with a dialysis bag of 0.8-10,000, and then the product is extracted with ether until no 9 is detected in the organic phase. -Anthral. The solution was then lyophilized. A water-soluble chitin pH probe with a substitution degree of 0.45% was obtained.

Embodiment 3

[0012] Example 3: Add 100 mL of 0.12 mg / mL 9-anthracenaldehyde methanol solution to 100 mL of 10 mg / mL water-soluble chitin solution with a deacetylation degree of 51%, stir the mixture for 8 hours, and then add 5 mg of cyano Sodium borohydride, continue to stir for 12 hours, and the reaction solution is rotatably evaporated, and the concentrated solution is soaked in deionized water for 3-4 days with a dialysis bag of 0.8-10,000, and then the product is extracted with cyclohexane until it cannot be detected in the organic phase. 9-anthral. The solution was then lyophilized. A water-soluble chitin pH probe with a degree of substitution of 0.8% was obtained.

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Abstract

The invention discloses a soluble chitose pH probe and preparation method used as the acidic organelle detection. The probe is made by amination and the soluble chitose is connected with the 9-anthracene aldehyde, so the substituted degree of the pH probe is 0.2%-1.8%. The method is to solve the chitose into the distilled water and add the 9-anthracene aldehyde under room temperature then the reductant; after removing the impurity by dialysis and extraction, the mixture is freeze dried to get the probe. The new fluorescent quanta point can avoid the toxic of the organic solvent, so the probe has the widely applied future in cell identification, biological marking and the illness diagnose.

Description

technical field [0001] The invention relates to a new water-soluble chitin pH probe used for acid organelle detection and a preparation method thereof, belonging to the field of chemistry. Background technique [0002] Fluorescent probes are widely used in cell biology and can be used to detect zinc ions and singlet oxygen in cells. Among the many objects of study inside the cell, the proton is one of the most important targets because pH plays a key role in the cell. Usually there are two pH ranges in cells, one is in the cell fluid, the pH range is 6.8-7.4; the other is in acidic organelles, such as lysosomes, the pH range is 4.5-6.0, and most of them are diseased cells. At present, there are commercial products for the probes used in the cell fluid, but there are few studies on the probes used in acidic organelles, but if the probes can be extended to acidic environments, it will be of great significance to the diagnosis of diseases in cells . Contents of the inventio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/52C12Q1/02
Inventor 杜予民庞知益李艳
Owner WUHAN UNIV
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