Purified thermostable Pyrococcus furiosus DNA polymerase I

Abstract

Purified thermostable Pyrococcus furiosus DNA polymerase that migrates on a non-denaturing polyacrylamide gel faster than phosphorylase B and Taq polymerase and more slowly than bovine serum albumin and has an estimated molecular weight of 90,000-93,000 daltons when compared with a Taq polymerase standard assigned a molecular weight of 94,000 daltons.

Description

CROSS REFERENCE TO RELATED APPLICATION

[0001] This application is a continuation-in-part application of application Ser. No. 07 / 779,846, filed Oct. 18, 1991, which is a continuation-in-part of application Ser. No. 07 / 657,073, filed Feb. 19, 1991, which is a continuation-in-part of application Ser. No. 07 / 620,568, filed Dec. 3, 1990.TECHNICAL FIELD

[0002] The present invention relates to a thermostable enzyme having DNA polymerase I activity useful in nucleic acid synthesis by primer extension reaction. BACKGROUND

[0003] The archaebacteria are a recently discovered group of microorganisms that grow optimally at temperatures above 80° C. Some 20 species of these extremely thermophilic bacteria-like organisms have been isolated, mainly from shallow submarine and deep sea geothermal environments. Most of the archaebacteria are strict anaerobes and depend on the reduction of elemental sulfur for growth.

[0004] The archaebacteria include a group of “hyperthermophiles” that grow optimally ...

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