Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Asymmetric PCR coupled with post-PCR characterization for the identification of nucleic acids

a nucleic acid and characterization technology, applied in the field of nucleic acid chemistry and nucleic acid identification, can solve problems such as more difficult to solve, and achieve the effect of increasing intensity

Inactive Publication Date: 2007-03-29
ROCHE MOLECULAR SYST INC
View PDF1 Cites 33 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Not only is this true in terms of differentiation between different infectious species, but is also true, and can be even more difficult to resolve, when trying to discriminate between closely related agents, e.g., different strains of a pathogen such as the subtypes of hepatitis C virus (HCV).

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Asymmetric PCR coupled with post-PCR characterization for the identification of nucleic acids
  • Asymmetric PCR coupled with post-PCR characterization for the identification of nucleic acids
  • Asymmetric PCR coupled with post-PCR characterization for the identification of nucleic acids

Examples

Experimental program
Comparison scheme
Effect test

example

Use of the Invention to Determine HCV Genotype

[0119] Determining the genotype of an HCV (hepatitis C virus) positive sample has important clinical significance and utility. Knowing the genotype of the specific virus allows physicians to select the correct treatment regimen. As explained above, the current invention can be used to discriminate amongst a number of nucleic acid targets (e.g., even ones that are closely related in sequence) such as HCV genotypes. The methods of the invention use an oligonucleotide probe that is specifically designed to be at least partially complementary to a region of target sequence divergence (e.g., a region that shows divergence between different strains, alleles, species, etc.) in conjunction with using the different Tms obtained from post-PCR melting and / or annealing analysis to distinguish the nucleic acid targets.

[0120]FIG. 5 shows aligned sequences of 6 different HCV genotypes (namely types 1a, 2a, 3a, 4, 5, and 6) along with an exemplary hyb...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
wavelengthaaaaaaaaaa
wavelengthaaaaaaaaaa
wavelengthaaaaaaaaaa
Login to View More

Abstract

The present invention provides methods, compositions, and kits for quantification and identification of target nucleic acid sequences, either in pure solutions or from mixtures of various nucleic acids. In other aspects, the invention provides compositions and methods for HCV genotyping.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS [0001] This application claims priority to and benefit of the following United States Provisional Patent Applications: [0002] Application Ser. No. 60 / 695,991, filed Jun. 30, 2005; [0003] Application Ser. No. 60 / 696,253, filed Jun. 30, 2005; [0004] Application Ser. No. 60 / 696,293, filed Jun. 30, 2005; and [0005] Application Ser. No. 60 / 696,303, filed Jun. 30, 2005. Each of these specifications are hereby incorporated by reference in their entirety.FIELD OF THE INVENTION [0006] The current invention relates to the fields of nucleic acid chemistry and nucleic acid identification. More specifically, the invention relates to methods and compositions for amplifying and classifying specific nucleic acid sequences, which can be used for such purposes as diagnostics. BACKGROUND OF THE INVENTION [0007] Numerous examples of the need for quick and reliable nucleic acid classification / identification exist, especially in fields such as medicine. For exampl...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(United States)
IPC IPC(8): C12Q1/68C12P19/34
CPCC12Q1/6818C12Q1/6823C12Q1/6851C12Q1/6858C12Q1/6883Y10S435/975C12Q1/707C12Q2565/101C12Q2563/173C12Q2527/107C12Q2561/113C12Q2531/107C12Q2561/101C12Q2523/319
Inventor NEWTON, NICKWILL, STEPHEN G.
Owner ROCHE MOLECULAR SYST INC
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com