Method for sterilization of biological preparations
a technology for biological preparations and preparations, applied in the field of sterilization of biological preparations, can solve the problems of wbc in a transfusion liquid, damage to biological materials during preservation, and damage to recipients, and achieve the effect of reducing the amount or activity
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Examples
example 1
The Effect of UV Exposure on the Survival of Lyophilized RBC
[0030]The effects of irradiation and freeze drying on red blood cells (RBC) were evaluated in this experiment. The freezing solution used was composed of 30% (w / v) dextran in PBS (Ca2+ and Mg2+ free). Packed RBC obtained from the Israeli Blood Services were mixed at a ratio of 1:1 (v / v) with the freezing solution. 2.5 ml of RBC solution was put in a 16 mm diameter of glass test tubes (Manara, Israel) which were then frozen. Freezing was done using the MTG freezing device (IMT, Israel) at a cooling rate of 1000Ā° C. / min; (thermal gradient) G=5.5Ā° C. / mm, V=3 mm / sec. The samples were also rotated at 56 RPM (rounds per minute).
[0031]After freezing, samples were put in a lyophilizer (Labconco, USA) for 3 days (condenser ā80Ā° C.). After 3 days of lyophilization, when the samples contained 10% or less of their original water content, one sample was placed in a Petri dish and exposed to UV radiation for 1 hour and the other was prot...
example 2
The Effect of UV Radiation on Lyophilized RBC Survival
[0033]In this experiment packed RBC were frozen with a freezing solution containing: 30% (w / v) dextran 40,000 Dalton and 0.47 mg / ml EGCG (Cayman Chemical, USA). The freezing solution and the packed RBC were mixed in a ratio of 1:1 (v / v). 2.5 ml of the cell suspension were put in 16 mm diameter glass test tubes (Manara, Israel). A total of 4 test tubes were frozen. The samples were frozen at a cooling rate of 1000Ā° C. / min; (thermal gradient) G=5.5Ā° C. / mm, V=3 mm / sec using the MTG Device (IMT, Israel). The samples were also rotated at 56 RPM.
[0034]After freezing, samples were placed in liquid nitrogen. After the passage of varying time periods (between Ā½ hour to a few weeks) samples were placed in a lyophilizer (Labconco, USA) with a condenser temp of ā80Ā° C.) for 72 hours, and the samples were dried such that they had the appearance of a powder and had less than 10% of their original water content. Then samples were transferred to...
example 3
The Effect of Partial Drying on RBC Survival
[0036]Fresh whole rat's blood (extracted from Sprague-Dawley rats) was washed once. Plasma was removed and the packed RBCs were suspended in a 1:3 ratio (v / v) with a freezing solution composed of 0.945 mg / ml EGCG and 20% (w / v) Dextran 40 kD in 0.9% (w / v) NaCl solution, and the final hematocrit was 25%. Three samples (2.5 ml each) were frozen each in a 16 mm diameter glass test tube (Manara, Israel) using the MTG device (IMT, Israel), with the following parameters: velocity=3 mm / sec; temperature gradient was 5.5Ā° C. / mm, the test tubes were rotated at 60 rpm. After freezing, samples were stored in LN until lyophilization. Lyophilization was done in a special lyophilization device (IMT, Israel) subject of co-pending PCT application No. IL2005 / 000124, which has a condenser temperature of ā190Ā° C. and samples were kept at a temperature of ā20Ā° C. Samples remained in the device for 48 hours. After 48 hours samples were taken out and thawed in a ...
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Abstract
Description
Claims
Application Information
- IPC
- C12N5/078; A61L2/10; A61L2/00; A61M1/16; C12N5/07
- CPC
- A61L2/0047; A61L2/0035
- Inventors
- NATAN, YEHUDIT; KANIAS, TAMIR