Maspin, Maspin Derivatives, and Maspin Mimetics for Reducing ROS, Inflammation, and Skin Aging

a technology of rosacea and rosacea, which is applied in the field of rosacea, rosacea, inflammation, and skin aging, can solve the problems of oxidative stress in the cell, and achieve the effect of reducing or eliminating at least one of the symptoms

Inactive Publication Date: 2016-05-26
NORTHWESTERN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The present disclosure provides methods for improving skin appearance by reducing wrinkles and improving elasticity. The methods can be applied to areas such as the face, eyes, mouth, arms, legs, back, stomach, etc. The patent also suggests that the methods can be used to reduce or prevent inflammatory conditions such as benign prostate hyperplasia, inflammatory bowel disease, ROS-induced obesity, diabetes, and atherosclerosis. The technical effects of the patent include improved skin appearance and reduced inflammation.

Problems solved by technology

Thus, excessive production and inadequacy in a normal cell's antioxidant defense system can cause the cell to experience oxidative stress.

Method used

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  • Maspin, Maspin Derivatives, and Maspin Mimetics for Reducing ROS, Inflammation, and Skin Aging
  • Maspin, Maspin Derivatives, and Maspin Mimetics for Reducing ROS, Inflammation, and Skin Aging
  • Maspin, Maspin Derivatives, and Maspin Mimetics for Reducing ROS, Inflammation, and Skin Aging

Examples

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example 1

Use of Maspin as an Anti-ROS Scavenger Against Cell Proliferation, Inflammation, and Aging

Experimental Procedures

[0041]Plasmid constructs and cell culture—Maspin X-ray crystal structure was reported by Law et al. (16) and Al-Ayyoubi et al. (17). Molsoft ICM-pro Version 3.48 was used to analyze the X-ray structure of human maspin (PDB ID: 1xu8), as reported by Law et al (16) to analyze the presence of exposed cysteine residues, which can serve as site for oxidation. QuikChange II site-directed mutagenesis kit (Stratagene) was used to mutate cysteine residues to serine residues in pEF-IRES-neo-h.maspin using the specific mutagenic primers (Table-S1). Mutations in the plasmid were confirmed by DNA sequencing.

[0042]Murine mammary tumor TM40D cells were used and maintained as described previously (18). TM40D cells were transfected with pEF-IRES-neo-h.maspin wild type (Mp), triple mutation (T) or control vector alone (Neo) by Effectent reagent (Qiagen). The stable transfectants were selec...

example 2

Maspin Interacts with Trx-1 in Redox Regulation

[0067]Maspin displays a conformational change from open to closed status around the G-helix region (Law et al., J Biol Chem (2005), herein incorporated by reference). Three structurally close cysteines in maspin protein (C20, and C287 or C373) are exposed in opened conformation, which are subject to ROS attacks and form disulfide bond within maspin molecule. In fact, we had previously observed the formation of maspin intramolecular disulfide bonds in human breast epithelial MCF10A cells (Nawata, et al., Int J Mol Med 27, 249-54 (2011), herein incorporated by reference). In biological systems, cytoplasmic disulfide bonds are generally taken care of by Trx1 (Nakamura et al, Antioxid Redox Signal 7, 823-8 (2005), herein incorporated by reference). To determine whether maspin disulfide bond interacts with Trx1, we first transfected flag-tagged cysteine mutated Trx1 (cysteine C35 is mutated to alanine) to the maspin-expressing MCF7 breast tu...

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Abstract

The present invention provides compositions, methods, and systems for treating inflammatory conditions (e.g., by inhibiting reactive oxygen species) in or on a subject with maspin, maspin derivatives, or maspin mimetics. In some embodiments, such agents are applied to the skin of a subject (e.g., to reduce skin aging).

Description

CROSS-REFERENCE TO RELATED APPLICATIONS[0001]The present application claims priority to U.S. Provisional Application Ser. No. 62 / 082,456, filed Nov. 20, 2014, which is herein incorporated by reference in its entirety.STATEMENT REGARDING FEDERAL FUNDING[0002]This invention was made with government support under CA 079736 awarded by the NIH. The government has certain rights in the invention.FIELD OF THE INVENTION[0003]The present invention provides compositions, methods, and systems for treating inflammatory conditions (e.g., by inhibiting reactive oxygen species) in or on a subject with maspin, maspin derivatives, or maspin mimetics. In some embodiments, such agents are applied to the skin of a subject (e.g., to reduce skin aging).BACKGROUND[0004]Oxidative stress, a hallmark of many tumors, inflammatory response, and cell aging, is caused by an imbalance between the generation of reactive oxygen species (ROS) and cells' ability to clear oxidants. Processes associated with proliferat...

Claims

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Application Information

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IPC IPC(8): A61K38/57A61Q19/08A61K9/00A61K8/64
CPCA61K38/57A61K8/64A61K2800/782A61K9/0014A61Q19/08
InventorZHANG, MING
OwnerNORTHWESTERN UNIV