Composition and method for the treatment of obesity

a technology for obesity and composition, applied in the field of composition and method for the treatment of obesity, can solve the problems of increased risk of heart attack and stroke in high-risk cardiac patients, potentially serious psychiatric disorders, etc., and achieve the effects of increasing the protective effect against dna, and reducing the risk of strok

Inactive Publication Date: 2016-10-13
PRASAD KODIMULE
View PDF1 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0116]It is well known hydroxyl radical (OH) is generated in human body via Fenton reaction. Since OH radical has extreme reactivity, it can easily damage DNA to produce malondialdehyde (MDA) and various oxidative lesions. MDA combines TBA (2-thiobarbituric acid) to produce TBARS (thiobarbituric acid reactive substances) which resent a maximum absorbance at 530 nm. On the other hand, as the oxidative lesions have no conjugative system in the molecules, they cannot be detected by a spectrophotometer at 530 nm. It means that these oxidative lesions can bring about no interference with the determination of MDA. Hence, the value of A532 can evaluate the amount of MDA, and ultimately reflect the extent of DNA damage. Based on the formula “protective effect”, it can be deduced that the decrease of A537 value indicates a protective effect against DNA damage. As seen in above graph when compared to Standard BHA, the composition dose dependently increased the protective effect against DNA, damage from 10-100 μg / mL. At 100 μg concentration the percentage protective effect of the composition and BHA was found to be 91.82% and 51.37% respectively. (Table 9, FIG. 11).

Problems solved by technology

Obesity is defined in terms of body mass index (BMI), which is calculated as body weight divided by square of height, and it is mainly a result of an imbalance between energy intake and expenditure.
Sibutramine and rimonabant were recently withdrawn from the market due to adverse side effects.
For example, Sibutramine could lead to an increased risk of heart attack and stroke in high risk cardiac patients, whereas the latter could lead to potentially serious psychiatric disorders (Ghazali et al., 2013).

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Composition and method for the treatment of obesity
  • Composition and method for the treatment of obesity
  • Composition and method for the treatment of obesity

Examples

Experimental program
Comparison scheme
Effect test

example 1

Preparation of Sunflower Material

[0066]100 kg of sunflower seed powder was taken into a cleaned vertical 1.0 KL extractor. The bottom of the extractor comprises of a perforated plate on which filtration cloth was fixed. The bottom of the extractor was connected to a transfer pump input and output of the transfer pump was connected to T bend. One end was connected to extractor top for circulation of extraction mass while extraction period and other end of T bend were connected to receiver tank.

[0067]The above mentioned mass was extracted with 7-8 bed volumes of demineralized water. Extraction was continued at 80-85° C. temperature about 7-8 hrs with continuous circulation of extract with transfer pump. After completion of extraction, filter the extract through 5 micron SS candle filter and collect clear extract in a receiver tank. The bed was re-extracted by adding 5-6 bed volumes of demineralized water 3 more times at 80-85° C. temperature about 7-8 hrs and filter through 5 micron S...

example 2

Spectrophotometric and HPLC Derivation

[0069]The material obtained under Example 1 was subjected to phyto-chemical derivation through spectrophotometric and HPLC estimations.

[0070]A. Derivation of Polyphenol Content by Folin-Ciocalteous Method

Standard Preparation:

[0071]Dissolve 100 mg of STD (99.9% pure) in 100 ml of volumetric flask by using 50% methanol solution (1000 ppm Chlorogenic acid stock standard). From this 1000 ppm stock standard prepare 30 ppm, 60 ppm, 90 ppm, 120 ppm, 150 ppm and 180 ppm standard solutions by diluting using 50% methanol solution.

Sample Preparation:

[0072]15-20 mg of the preparation from Example 1 was placed into 100 ml of volumetric flask, about 50 ml of 50% methanol solution was added and the mixture sonicated for 5 mins, then diluted to 100 ml with 50% methanol, then further diluted to 5.0 ml of above solution to 10 ml using 50% methanol.

[0073]Prepared a series of test tubes (one for blank, each STDs and samples) each containing 15 ml of 50% methanol so...

example 3

Toxicity Study

[0097]The Ames test is widely used in the determination of possible gene mutations by various agents. A positive response in any single bacterial strain either with or without metabolic activation is sufficient to designate a substance as an antimutagen. It is estimated that 90% of all carcinogens also are mutagens, and with this figure in mind, Bruce Ames and his colleagues developed a test in the 1970s that uses special bacteria that are very sensitive to mutagenic agents. The Food and Drug Administration (FDA) now uses the Ames test to screen many chemicals rapidly and inexpensively. Those few chemicals that appear to be mutagenic by the Ames test are tested further in animals to assess their ability to cause cancer.

[0098]The Salmonella mutagenicity test was specifically designed to detect chemically induced mutagenesis both in presence and absence of metabolic activation. This assay helps to identify substances that can produce genetic damage that leads to gene mut...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
thicknessaaaaaaaaaa
thicknessaaaaaaaaaa
Login to view more

Abstract

A composition formulated from sunflower, including Helianthus annulus and methods for use of the composition for treating obesity in a subject.

Description

FIELD OF THE INVENTION[0001]The invention generally relates to a composition formulated from sunflower and methods for the use of the composition in the treatment of obesity and obesity management.BACKGROUND[0002]Sunflowers are botanically classified as in the genus Helianthus belonging to family compositae. They are a large plant and are grown throughout the world because of their relatively short growing season. Domesticated sunflowers typically have a single stalk topped by a large flower. This is significantly different from the smaller, multiply branched wild sunflower. During the growing season, the individual flowers are each pollinated. The sunflower plants reach various heights, but most are from 1.52-2.1 meters tall. Seed development begins moving from the outer rim of the flower toward the center. It generally takes 30 days after the last flower is pollinated for the plant to mature (Agribusiness Handbook, 2010).[0003]Sunflowers, which are used mainly as an ornamental cro...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(United States)
IPC IPC(8): A61K31/216A61K36/28
CPCA61K36/28A61K31/216A61K31/7032A61K2300/00
Inventor PRASAD, KODIMULE
Owner PRASAD KODIMULE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap