Modified collagen
a technology of modified collagen and collagen dispersion, which is applied in the direction of prosthesis, peptide/protein ingredients, antibacterial agents, etc., can solve the problems of difficult to manufacture collagen-based membranes or lyophilized sponges at commercial scale, inefficient and costly at commercial scale, etc., to facilitate the efficient manufacture of collagen-based materials and improve the potential effectiveness of those materials
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example 1
Collagen Isolation
[0105]Collagen can be isolated from a number of sources, for example, animal hides and animal tendons. In a preferred embodiment, the collagen is isolated from animal tendon, for example equine or bovine tendon; although any known source of collagen, including fibrous tissue, optionally connective tissue, may be used and selected by one skilled in the art. Preferably, the collagen is isolated from equine tendon. In the method of isolation, equine tendons were milled to degrade the collagen source. The milled equine tendons were treated with a number of reagents, including 1N sodium hydroxide (NaOH) to remove microbiological contamination such as prions at the beginning of the process. Treatment steps with hydrogen peroxide and washing steps at different pH values were conducted, followed by a milling step, which was used to increase the surface for the next treatment step. The molecular weight of the collagen source was additionally reduced by treatment with the pr...
example 2
Compounding Process and Equipment
[0110]An aqueous modified collagen dispersion was prepared in a stainless steel vessel using pre-heated (35-42° C.) purified water, which was adjusted to pH 4.0±0.2. High shear mixing was required to break up the modified collagen mass and expose the collagen fibres to the acidic medium. The high shear mixer (homogeniser) comprised a rotor / stator head that is designed to create high shear forces by pulling the modified collagen through the rotating homogeniser head and forcing the modified collagen against the proximal stationary stator head. It is this design that provided the high shear forces required to separate the fibrous collagen mass at the beginning of the aqueous dispersion preparation. However, other comparable mixing equipment may also be used; and can be selected by one skilled in the art. For example, an IKA Ultra-Turrax mixer may be used at a high speed for about 2 to about 5 minutes.
[0111]If required, although not essential, the resul...
example 3
Characterisation
[0116]All compositions (membranes) were prepared from a 0.6% dispersion using the method described herein above. All tests on the collagen dispersion were conducted within 1 day after compounding; and all characterisation experiments with the membranes were performed within 1 month after membrane manufacture using unsterilised membranes.
[0117]Dispersion Viscosity
[0118]The viscosity values of 0.9% collagen dispersions prepared from each of the fresh collagen, frozen collagen, non-matured lyophilised milled collagen, and matured lyophilised milled collagen according to Example 2 were measured using a Brookfield viscometer (Digital Rheometer DV-III+ with associated TC-501 Circulating Bath). The viscosity values were measured at a constant shear rate (15 s−1) and over a temperature range from 25 to 40° C. at 5° C. increments. 60 measurements per temperature were averaged to obtain reliable results.
[0119]The dispersion viscosity depends on the temperature and decreases wh...
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