Methods and compositions comprising steroid honey bee feeding inhibitors
a technology of honey bees and inhibitors, applied in the field of new honey bee feeding inhibitors, can solve the problems of difficult to overstate the role of bees in the commercial production of food, slow progression of symptoms, complex reasons for increasing colony collapse, etc., to prevent pesticides and insecticides from adversely affecting honey bee colonies, and maintain the health of honey bee colonies and other issues, to prevent pesticides and insecticides from being used more effectively. , to preven
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[0087]We have found that two classes of steroidal compounds that inhibit foraging by honey bees, Apis mellifera. The structure of two of the compounds that were tested is shown below. 20-hydroxyecdysone is the molting hormone of most insects and can be produced by plants as a phytoecdysteroid. Cucurbitacin B is a steroid produced primarily by members of the family Cucurbitaceae. Neither of these compounds have been tested as honey bee feeding inhibitors.
[0088]
Assessing Bee Repellency
[0089]To test compounds we conducted foraging assays at the ISU Horticulture research farm. A grid of 20 stands were placed about 50 m from 6 honey bee hives. Modified pan traps (referred to as ‘Bee bowls’) were placed on these stands so they were 20 cm from the ground. Holders were spaced 40 cm apart in an array of 4×5. Bee bowls, painted yellow, contained a cotton ball soaked in corn syrup placed in the center to attract foraging bees. To monitor foraging behavior, we counted the number of bees visitin...
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