An apparatus and method for automated
microdissection of tissue from slides to optimize
tissue harvest from regions of interest are disclosed. The apparatus includes at least a processor and a memory communicatively connected to the at least a processor, wherein the memory contains instructions configuring the at least a processor to receive a stained input slide, identify a
region of interest on the stained input slide, generate a segmentation map of the
region of interest as a function of a segmentation
algorithm, register a segmented
region of interest, as a function of the segmentation map, onto an unstained slide, wherein registering the segmented region of interest includes determining an orientation of the unstained slide corresponding to the segmented region of interest of the stained input slide, recording the orientation of the unstained slide relative to a
reference plane, and registering the segmented region of interest to the unstained slide.