A waterproof gate assembly structure is constituted by a sheathing layer, a nozzle, an inflatable inner casing, iron pieces, and screw-holes, wherein the sheathing layer is fixed on a wall by transfixing fixing screws into the screw-holes, small holes, and pads, and an exterior of the sheathing layer is provided with the nozzle which is extended to the inflatable inner casing in the sheathing layer. Gas can be filled into the inflatable inner casing through the nozzle, until it is full, such that an unfilled space can be more tightly filled, thereby achieving an effective waterproof function. In addition, the sheathing layer is also provided with locking elements for taking out and replacing the inflatable inner casing.