The invention discloses a rape seed soaking agent and a preparation method and application. The rape seed soaking agent is prepared from indoleacetic acid, gibberellin, brassinolide, monopotassium phosphate and zinc sulfate in a certain proportion. The method includes the steps that firstly, gibberellin, indoleacetic acid and brassinolide are weighed at normal temperature, gibberellin, auxin and brassinolide are fully mixed and dissolved with ethyl alcohol, water is added, and a mixed solution a of the three kinds of powder is obtained; secondly, monopotassium phosphate and zinc sulfate are weighed at normal temperature, water is added into monopotassium phosphate and zinc sulfate, monopotassium phosphate and zinc sulfate are dissolved, and a mixed solution b of the two kinds of powder is obtained; thirdly, the mixed solution a, prepared in the first step, of the three kinds of powder and the mixed solution b, prepared in the second step, of the two kinds of powder are evenly mixed at normal temperature, volume determination is carried out, and the rape seed soaking agent is obtained. Cost is low, the method is easy to use, operation is easy and convenient, cost is low, germination is quick, seedling emergence is orderly, growth is good, the seedling rate is high, the quality of rape is improved, the rape seed soaking agent has an obvious yield increase effect, and the yield of the rape is increased by 8-12%.