Bacteria strain (X-7 strain) for culturing bait eelworm and its eelworm culture method

A culture method and technology of nematodes, applied in the direction of bacteria, application, animal feed, etc., can solve the problems of large-scale cultivation of difficult biological bait, liquid culture is not as convenient as bacteria, etc., and achieve the goal of promoting industrialized cultivation, excellent biological bait, and easy cultivation Effect

Inactive Publication Date: 2008-09-03
GUANGDONG ENTOMOLOGICAL INST
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  • Summary
  • Abstract
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AI Technical Summary

Problems solved by technology

However, in commercial application, since Escherichia coli is a human pathogen, it is difficult to be used for large-scale cultivation of biological bait; although yeast is safe as a biological bait, the cultivation of yeast, especially liquid cultivation, is not as good as Bacteria are convenient

Method used

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Examples

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Embodiment Construction

[0012] The following experiments and operating examples are further illustrations of the present invention, and should not be regarded as limiting the present invention.

[0013] 1. Bacterial strain Xenorhabdus sp.X-7 CCTCC M 206090 is contained on the peptone water culture medium of 1% (mass percentage) peptone, 0.5% (mass percentage) sodium chloride, cultivates the bacterium liquid that obtains Xenorhabdus genus at 26 ℃ ;

[0014] 2. The nematode medium was sterilized at 121° C. for 40 minutes. The composition ratio (by mass percentage) of the nematode medium was: yeast extract 1%, flour 10%, soybean powder 5%, lard 4%, water 65%, sponge 15%;

[0015] 3. Add 100 grams of sterilized nematode culture medium to a 500ml Erlenmeyer flask, then add the cultured Xenorhabdus bacterial solution, then insert sterile P.redivivus nematodes, and culture at 25-28°C , Harvest the cultured nematodes after 6 to 15 days, the nematodes in the culture can be harvested.

[0016] When doing th...

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Abstract

The invention discloses a bacterium strain (X-7 system) to culture bait nematolog and nematolog culturing method, which comprises the following steps: culturing Xenorhabdus sp. X-7 CCTCC M 206090 on the culture medium with peptone water under 25-28 deg. c to obtain the bacterial liquid; adding nematolog culture medium; switching in Panagrellus redivivus; culturing under 25-28 deg. c for 6-15h; obtaining the product as bait in the aquatic product culturing industry.

Description

technical field [0001] The invention relates to a bacterium for cultivating bait nematodes and a method for cultivating the bait nematode Panagrellus redivivus. Background technique [0002] World-wide aquaculture, especially the aquaculture of prawns, has promoted the development of aquatic product seedling cultivation, and also intensified the demand for biological bait, especially the demand for Artemia. Artemia is generally collected from high-salinity waters, and its intensive farming has not yet formed a scale. Initially, the Great Salt Lake in Utah, USA was a place rich in Artemia. Over the years, the production of Artemia eggs has always accounted for more than 80% of the world's production, controlling the supply of the world market. However, since the 1990s, due to the over-harvesting of Artemia resources and changes in the salinity of the lake, the production of Artemia in salt lakes has declined sharply, resulting in high prices for Artemia. Central Asia also s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K1/18A23K10/18A23K50/80
CPCY02A40/818
Inventor 韩日畴丘雪红曹莉刘小芬刘秀玲陈镜华
Owner GUANGDONG ENTOMOLOGICAL INST
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