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Streptomycin immune chromatographic semiquantitative test paper detecting method

A detection method, immunochromatography technology, applied in the field of detection of antibiotic residues, can solve problems such as incomplete detection technology of streptomycin

Inactive Publication Date: 2009-01-07
SOUTH CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the corresponding streptomycin detection technology in my country is not very complete at present, and it is necessary to study a simpler, faster and more convenient detection method

Method used

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  • Streptomycin immune chromatographic semiquantitative test paper detecting method

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Embodiment Construction

[0023] (1) Test paper in the form of 1 detection line + 1 reference line

[0024] The detection line is coated with streptomycin monoclonal antibody or streptomycin detection antigen at a concentration of 0.1-30 μg / mL, and the width is 1mm; the reference line is coated with an anti-second species at a concentration of 0.01-30 μg / mL One IgG line of animal protein, 1mm wide; the distance between two lines is 2-6mm. When the color of the detection line is the same as that of the reference line, the sample is negative; when the color of the detection line is lighter than the reference line, the sample is positive, and the concentration is greater than Aμg / kg, A is the set detection limit, which can be greater than or equal to A certain concentration of 10 μg / kg. The reference line is always in color, if the reference line does not show color, it indicates that the test paper is invalid.

[0025] (2) Test paper in the form of 2 detection lines + 1 reference line

[0026] Two detec...

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Abstract

The present invention relates to a streptomycin immunochromatographic semiquantitative test paper detection method. It is characterized by that on the back lining of said test paper are successively stuck sample liquor absorption portion, colloidal gold labeling portion, detection reaction portion and water-absorbing portion. Said invention also provides the concrete structure composition of said detection reaction portion and the concrete application method of said test paper.

Description

technical field [0001] The invention relates to a method for detecting antibiotic residues in food, in particular to a method for detecting streptomycin semi-quantitative test paper. Background technique [0002] Antibiotic residue is a common problem in livestock and poultry farming all over the world, and streptomycin is a relatively common residual antibiotic. Streptomycin is an aminoglycoside antibiotic, which plays an important role in the treatment of livestock Gram-negative bacteria and Mycobacterium tuberculosis infection, but its residues have relatively large toxic and side effects. In order to strengthen the management of veterinary drug residues and ensure the quality and safety of livestock products, the Ministry of Agriculture of my country revised the "Maximum Residue Limits of Veterinary Drugs in Animal Foods" in 2002, which stipulates that the maximum residue limit of streptomycin in milk is 200 μg / kg, which is harmful to cattle. There are also corresponding...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/558G01N33/543G01N21/78G01N33/52
Inventor 孙远明雷红涛黄晓钰王弘吴青谌国莲潘科肖治理
Owner SOUTH CHINA AGRI UNIV