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Method for quick filtering out bacteria having characterization of adsorption for degrading polycyclic aromatic hydrocarbon

A technology of polycyclic aromatic hydrocarbons and adsorption characteristics, which is applied in the field of microbial remediation of contaminated soil, and can solve problems such as difficult screening

Inactive Publication Date: 2009-04-15
SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main disadvantage of this method is that it only screens the microbial groups that grow rapidly in the suspension liquid, and it is difficult to screen the degrading bacteria with adsorption capacity, and most of the pollutants in the polluted environment are adsorbed on solid particles

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0029] The present invention takes petroleum-contaminated soil as the research object, and pyrene with a four-ring structure is used as a representative polycyclic aromatic hydrocarbon, and the specific operation steps are as follows:

[0030] 1. Soil sample collection and processing

[0031] Take the 0-20cm soil layer of oil-contaminated soil, sieve it through a 1mm sieve, and store it at 4°C.

[0032] 2. Enrichment and isolation of degrading bacteria using adsorbent carriers

[0033] Wrap 15 g of the adsorptive carrier with gauze, place it in a Erlenmeyer flask for sterilization (121°C damp heat sterilization for 20 minutes), then spray 50 uL of 1 mg / mL pyreneacetone solution on the carrier, let it stand for 2 hours, and let the acetone volatilize naturally. Add 40 mL of inorganic salt liquid medium and 10 mL of soil suspension, and culture on a shaker at 30°C in the dark. Every 10 days, 3-4 g of the carrier was transferred to a freshly prepared carrier-containing inorgani...

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Abstract

This invention relates to a novel method for screening polycyclic aromatic hydrocarbon degrading strains by using adsorption carrier. Polycyclic aromatic hydrocarbons (4 or higher) have low water solubility, and can be adsorbed onto solid particles whose surface microenvironment may contain polycyclic aromatic hydrocarbon degrading strains. This invention provides a rapid and highly efficient method for screening polycyclic aromatic hydrocarbon degrading strains with adsorption characteristic. The method utilizes adsorption carrier to culture and separating polycyclic aromatic hydrocarbon degrading strains, and double-layered flat plat technology to primarily screen, and screens polycyclic aromatic hydrocarbon degrading strains according to the degradation efficiency. The combination of primary and final screening can largely increase the screening efficiency.

Description

technical field [0001] The invention relates to the technical field of microbial remediation of polluted soil, in particular to a new method for screening polycyclic aromatic hydrocarbon-degrading bacteria by using an adsorptive carrier. Background technique [0002] Polycyclic aromatic hydrocarbons are a large class of environmental organic pollutants, which are carcinogenic, teratogenic, and mutagenic. Among them, 16 are listed as priority pollutants by the US Environmental Protection Agency. Eliminating the pollution of polycyclic aromatic hydrocarbons is of great significance for improving the environment and improving the quality of life of the people. The polycyclic aromatic hydrocarbons in the environment are mainly degraded by microorganisms, so the screening of efficient degrading bacteria is a key to the bioremediation technology of polycyclic aromatic hydrocarbon-contaminated soils. At present, the traditional method is to use liquid shake flask culture method to...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/00C12N1/26B09C1/10
Inventor 张颖王荣任瑞霞徐慧李慧史荣久
Owner SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI