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A method for evoking yili caladium polyploid under culture in vitro

A technology for in vitro cultivation of Fritillaria ilii, applied in horticultural methods, botany equipment and methods, plant cells, etc., can solve the problems of resource destruction, distribution area reduction, low reproduction coefficient, etc., to ensure mutagenesis efficiency, reduce workload effect

Inactive Publication Date: 2010-01-27
XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, artificially cultivated Fritillaria Ili has the problems of low reproduction coefficient and quality degradation. Wild Fritillaria Ili has been excavated in large quantities for a long time, its distribution area is shrinking day by day, and resources are seriously damaged. Therefore, the research on new germplasm resources of Fritillaria Ili are getting more and more attention

Method used

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  • A method for evoking yili caladium polyploid under culture in vitro
  • A method for evoking yili caladium polyploid under culture in vitro
  • A method for evoking yili caladium polyploid under culture in vitro

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] a. Rinse the surface of the fresh bulbs of Fritillaria Ili with tap water, dry the large bulbs in the sun for 12 hours, and dry the small bulbs for 8 hours, then disinfect the bulbs on the ultra-clean workbench with 75% alcohol by volume for 30 seconds, mass volume percentage Sterilize with 0.1% mercuric chloride for 6 minutes, treat with 15% hydrogen peroxide for 30 minutes, rinse with sterile water twice;

[0034] b. Cut the treated bulb into 0.1-0.2cm in step a 3 The size is inserted into the solid medium MS+KT 1.0mg / L+NAA 0.3mg / L for adventitious bud induction and proliferation, the temperature is 20±1°C, the light is 2000lx, and the time is 18h / d for cultivation;

[0035] c. After 40 days, 5-10 adventitious buds can grow from each bulb cut in step b, and these adventitious buds are cut into single buds, and 40 single buds are randomly taken and soaked in a solution containing 50mg / L colchicine, 2% In the liquid MS medium of dimethyl sulfoxide, soak on a shaker wit...

Embodiment 2

[0041] a. Rinse the surface of the fresh bulbs of Fritillaria Ili with tap water, dry the large bulbs in the sun for 18 hours, and dry the small bulbs for 10 hours, then treat the bulbs with 1% potassium permanganate aqueous solution on the ultra-clean workbench for 1 hour, 3% Sodium hypochlorite aqueous solution was treated for 10 minutes, 15% hydrogen peroxide was treated for 45 minutes, and sterile water was rinsed 3 times;

[0042] b. Cut the treated bulb into 0.1-0.2cm in step a 3 The size is inserted into the solid medium MS+KT 0.5mg / L+NAA 0.1mg / L for adventitious bud induction and proliferation, the temperature is 20±1°C, the light is 2000lx, and the time is 18h / d for cultivation;

[0043] c. After 40 days, 5-10 adventitious buds can grow from each bulb cut in step b, and these adventitious buds are cut into single buds, and 40 single buds are randomly taken and soaked in a solution containing colchicine 400mg / L, 2% In the liquid MS medium of dimethyl sulfoxide, soak o...

Embodiment 3

[0049] a. Rinse the surface of the fresh bulbs of Fritillaria Ili with tap water, dry the large bulbs in the sun for 24 hours, and dry the small bulbs for 12 hours, then soak the bulbs in 84 disinfectant on the ultra-clean workbench for 20 minutes, and sterilize them with 0.1% mercury liter 10min, 15% hydrogen peroxide treatment for 60min, rinse with sterile water 3 times;

[0050] b. Cut the treated bulb into 0.1-0.2cm in step a 3 The size is inserted into the solid medium MS+KT 0.8mg / L+NAA 0.4mg / L for adventitious bud induction and proliferation, the temperature is 20±1°C, the light is 2000lx, and the time is 18h / d for cultivation;

[0051] c. After 40 days, 5-10 adventitious buds can grow from each bulb cut in step b, and these adventitious buds are cut into single buds, and 40 single buds are randomly taken and soaked in a solution containing colchicine 1000mg / L, 2% In the liquid MS medium of dimethyl sulfoxide, soak on a shaker with a rotating speed of 100r / min for 48h; ...

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Abstract

The invention relates to a polyploid inducement method of Fritillaria pallidiflora Schrenk in vitro culture, which uses fresh bulb to be induced to produce adventitious buds. Inducement treatment is applied through cultivating in MS liquid medium with different density of colchicine to produce Fritillaria pallidiflora Schrenk polyploid plant. Bulb yield and medicinal components content of the plant are improved by changing chromosome ploidy to produce plant with good characteristics. Therefore, the problem of variety degeneration of Fritillaria pallidiflora Schrenk in a long-term course of Fritillaria pallidiflora Schrenk planting is overcomed. The method has the advantages that Fritillaria pallidiflora Schrenk polyploid can be obtained quickly with effectively improved content of active components and a shorter planting cycle.

Description

technical field [0001] The invention relates to the field of medicinal plant breeding, in particular to a polyploid induction method of Fritillaria ilii. Background technique [0002] Fritillaria Pallidiflora Schrenk, a perennial herb of the genus Fritillaria L. in the family Liliaceae, is used as medicine with its bulbs. Because the content of alkaloids in Ili Fritillaria is relatively high in the same kind of Fritillaria, and the price of medicinal materials is relatively low, it has attracted people's attention in recent years. Ili fritillary is planted in Xinjiang and the mainland, and has become an important Chinese herbal medicine and economic income-generating crop. However, artificially cultivated Fritillaria Ili has the problems of low reproduction coefficient and quality degradation. Wild Fritillaria Ili has been excavated in large quantities for a long time, its distribution area is shrinking day by day, and resources are seriously damaged. Therefore, the researc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/08A01H1/04A01H4/00C12N5/04
Inventor 王晓军刘传军郝秀英赵民安李晓瑾努尔波拉提刘敏康喜亮徐琴
Owner XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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