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Quinoa mutant construction method based on EMS induction

A construction method, quinoa mutation technology, applied in the field of EMS mutagenesis, can solve the problems of easily damaged seeds, time-consuming and labor-consuming, and easy germination of quinoa after soaking, so as to achieve efficient use of land resources, slow down the germination rate, and improve planting efficiency effect

Active Publication Date: 2019-11-15
SHANDONG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The inventor believes that the problems that must be overcome in the quinoa EMS mutagenesis method are: (1) overcome the problem that quinoa is soaked and easy to germinate
Quinoa seeds are very easy to germinate, which leads to the use of traditional plant mutagenesis methods, which will cause seeds to germinate during the mutagenesis shock process, and the germinated seeds are extremely vulnerable to damage during the shock process, which in turn affects the final mutagenesis library construction
(2) overcome the insufficient problem that EMS reagent contacts with quinoa seed
However, quinoa seeds cannot be planted by spraying, and the method of clamping is time-consuming and labor-intensive, which is not the best solution

Method used

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  • Quinoa mutant construction method based on EMS induction
  • Quinoa mutant construction method based on EMS induction
  • Quinoa mutant construction method based on EMS induction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] S1: M 0 seed preparation,

[0057] Firstly, 1,000 plump quinoa seeds were selected and weighed, and the operation was repeated 3 times to obtain an average thousand-grain weight of 0.3 g of quinoa, and then a total of 6 g of seeds, about 20,000 seeds, were weighed and placed evenly in a 500ML Erlenmeyer flask. Approximately 2000 seeds per bottle.

[0058] S2: M 0 Seed mutagenesis pre-treatment

[0059] Add 10 times the volume of Tween-20 (Tween-20) aqueous solution containing 0.2% in the bottle, and shake it at 180 rpm for 30 minutes at room temperature. Take a 10cm×10cm filter membrane with a pore size of 40 meshes, wrap the mouth of the conical flask to prevent the seeds from flowing out, and wash it with water 5 times to remove excess Tween-20 aqueous solution.

[0060] S3:M 0 seed mutagenesis treatment

[0061] After putting on the protective clothing, place the Erlenmeyer flasks treated with Tween-20 in a fume hood, and after opening the ventilation, add 5 ti...

Embodiment 2

[0067] Comparison of different mutagenesis treatments:

[0068] (1) Seed germination comparison table for 12 hours of shaking culture at room temperature 24°C and 12 hours of shaking culture at low temperature 5.6°C:

[0069] Table 1

[0070] room temperature low temperature avoid light 40％ 8.7％ not dark 62.3％ 10％

[0071] (2) Comparison of different quinoa pretreatment schemes:

[0072] During the mutagenesis process, the plant seeds are usually pretreated by soaking, increasing the contact between the mutagen and the seeds, and directly soaking in water for about 10 hours. The operation is simple and convenient, but because quinoa is very easy to germinate, this method is not conducive to subsequent mutagenesis operate. The use of detergent is an effective alternative method. Detergents are divided into ionic detergents with strong effects, such as SDS, sodium lauryl sarcosinate, etc., which have strong effects. Commonly used non-ionic dete...

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Abstract

The invention belongs to the technical field of EMS mutation, and particularly relates to a quinoa mutant construction method based on EMS induction. Quinoa is a crop with good economic benefits. At present, the outcrossing rate of quinoa breeding is relatively high, breeding work is not complete, and construction of a stable mutant material can guarantee the breeding work of quinoa. The quinoa mutant construction method based on EMS induction is provided and aims to solve the problems that quinoa seeds are easy to damage in the process of vibration and easy to germinate after immersion and saponins interfere with the surfaces of the seeds. According to the technical scheme, the seeds are soaked in an EMS solution at low temperature after being cleaned with a Tween-20 aqueous solution. TheTween-20 is used for cleaning the surfaces of the seeds to solve the problem of easy germination of the seeds at low temperature, in combination with a planting plan, mutants can be obtained efficiently, and the planting efficiency is ensured.

Description

technical field [0001] The disclosure belongs to the technical field of EMS mutagenesis, and in particular relates to a method for constructing a quinoa mutant based on EMS induction, a quinoa mutant obtained by the method, and the application of the two in quinoa breeding. Background technique [0002] The information disclosed in this Background section is only intended to increase the understanding of the general background of the disclosure, and is not necessarily to be taken as an acknowledgment or any form of suggestion that the information constitutes prior art that is already known to those skilled in the art. [0003] Quinoa is a dicotyledonous plant of the genus Chenopodium in the family Amaranthaceae. It has many advantages such as high nutritional value, strong resistance to stress, and high yield per unit area. Quinoa has become an important crop to deal with international food security issues. During nearly 7,000 years of artificial domestication and cultivatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/06
CPCA01H1/06
Inventor 马长乐郑玲王萍萍邵群赵一伍
Owner SHANDONG NORMAL UNIV
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