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Quick cultivating method for low phytic acid crop

A technology for planting acids and crops, applied in the fields of botanical equipment and methods, plant regeneration, plant products, etc., which can solve problems such as heavy workload

Inactive Publication Date: 2007-08-08
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the need for one-to-one correspondence with the remaining seeds, the workload is very large

Method used

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  • Quick cultivating method for low phytic acid crop

Examples

Experimental program
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Embodiment 1

[0023] Embodiment 1: Rice

[0024] In 2005, the dry seeds of rice variety Topaz B were treated with cobalt-60 gamma rays in the irradiation center of Zhejiang University, the dose was 300Gy, the dose rate was 6Gy / min, and the treatment time was 50min, and M 1 generation seeds. m 1 About 200 grams of seeds were soaked, germinated, sown, and transplanted according to conventional techniques, and planted in the Jiande City Experimental Base of Zhejiang University. Harvest the seeds in October 2005 namely M 2 Substitute seeds are about 1000 grams.

[0025] Will M 2 The substitute seeds are cut in half horizontally, one half with embryos and the other half without embryos, corresponding one by one. The half without embryo was tested for inorganic phosphorus content.

[0026] The specific operation for determining the content of inorganic phosphorus is as follows: put half a seed (shelled seed, such as rice that needs to be shelled) in the hole of a V-type enzyme labeling plat...

Embodiment 2

[0028] Example 2: Corn

[0029] In October 2004, the dry seeds of maize inbred line Danhuang 25 were treated with cobalt-60 gamma rays at the irradiation center of Zhejiang University. The dose was 150Gy, the dose rate was 10Gy / min, and the treatment time was 15min. M 1 generation seeds. m 1 500 seeds were sown according to conventional techniques, and were planted in Lingshui Experimental Base, Hainan. When flowering, they were bagged and self-bred by individual plants. The seeds harvested in March 2005 were M 2 Substitute seeds are about 1500 grams.

[0030] Will M 2 The substitute seeds are cut in half horizontally, one half with embryos and the other half without embryos, corresponding one by one. The half without embryos was tested for inorganic phosphorus content. The specific operation of inorganic phosphorus content determination is the same as embodiment 1. The samples with light blue to dark blue color were HIP candidate kernels, and 6 dark blue samples were ob...

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Abstract

The invention relates to a method for quickly planting low-acid crop, which comprises that 1, planting induced M1 generation seed, harvesting the M2 generation seed; 2, on the part of M2 generation seed without blast is sampled to test the inorganic phosphor content of M2 generation seed to select out the seed with high content; 3, accelerating germination of left part with blast, planting to obtain the M2:3 generation seed; 4, measuring the inorganic phosphor content of M2:3 generation seed to find the change of low-acid body. The invention can shorten the time that inducing object into low-acid one, with wide application.

Description

technical field [0001] The invention relates to the field of breeding of new crop varieties, in particular to a method for quickly cultivating low-phytic acid crops. Background technique [0002] Phytic acid is the main form of phosphorus (P) in mature grains of crops, and it is easy to combine with Zn 2+ , Fe 3+ 、K + and Ca 2+ Chelation of metal ions. Phytic acid and phytate are practically not absorbed by humans and non-ruminants. Phytic acid is generally considered to be an important anti-nutritional component in cereal grains and one of the important causes of environmental P eutrophication. Therefore, cultivating crops with low phytic acid has dual functions of nutrition and environmental protection. [0003] U.S. Patent No. 6,111,168 discloses a technology for breeding new varieties of low-phytic acid crops. First, it uses induced mutation technology to create mutant populations and mix M. 1 M on plants 2 Seed, single plant M 2 plants. m 2 The seeds of the pl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/04A01H1/06A01H4/00A01H5/10
Inventor 舒庆尧吴殿星
Owner ZHEJIANG UNIV
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