Cynoglossus semilaevis ovum miosis gynogenesis method induced by bass frozen sperm

Abstract

The invention relates to a method for using the frozen sperm of bass to induce the cultivation of sliding-tongue ovum thelykaryon, which comprises that: using the bass sperm frozen in the liquid nitrogen, to induce the thelykaryon cultivation of sliding-tongue ovum; defreezing the sperm in 37Deg.C water bath, taking 100muL sperm to dilute the MPRS for 10 times, to be arranged in the cultivate container at 6Deg. C; using 1000muj / cm2 ultraviolet to radiate for 0.2min, to inactivate the sperm; then using the inactive sperm to induce the fecundation with the ovum; in 5min of fecundation, using 2Deg. C water bath to shock the zygote, while the shock time is 20min; arranging the blast into the sea bath at 23Deg. C to be cultivated. The invention has low cost.

Description

technical field [0001] The technology belongs to the field of marine biotechnology, and is a breeding technology for cultivating all-female, fast-growing new breed of half-smooth tongue sole, which can be directly applied to the seawater fish farming industry. Background technique [0002] Semi-smooth tongue sole is a unique economic fish distributed in the Yellow Sea and Bohai Sea of ​​my country. After artificial domestication and breeding in recent years, it has been developed into a rare fish in seawater factory culture. The body weight is more than 4 times that of the males, and about 50% of the male fish in the breeding fry grow slowly, and it is difficult to reach the size of commercial fish, which increases the cost of breeding and production, and restricts the large-scale breeding of fry. Female fry have important practical significance for improving the economic benefits of farming and expanding the scale of farming. Gynogenesis is a type of parthenogenesis, which r...

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention: the present invention is mainly aimed at the problem of slow growth of the male half-smooth tongue sole. In breeding, the weight of the 2-year-old female fish can reach more than 600 grams, while the male is only about 150 grams. The female fish can reach the standard of commercial fish , while the male fish cannot meet the commercial fish specifications, but the male and female fry cannot be distinguished during the fry sale period (5-10cm), and half of the male fry purchased by farmers cannot be sold after one year of breeding, resulting in an increase in breeding costs , in view of this reason, the sales volume of semi-smooth tongue sole fry has declined, and it is difficult to further expand the breeding scale