Cynoglossus semilaevis ovum miosis gynogenesis method induced by bass frozen sperm

A technique for the development of semi-smooth tongue sole and gynogenesis, which is applied in the field of marine biology, can solve the problems of difficult breeding scale, slow growth rate of semi-smooth tongue sole males, rising breeding costs, etc., and achieves the effects of high technical difficulty and improved flexibility.

Inactive Publication Date: 2007-08-08
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention: the present invention is mainly aimed at the problem of slow growth of the male half-smooth tongue sole. In breeding, the weight of the 2-year-old female fish can reach more than 600 grams, while the male is only about 150 grams. The female fish can reach the standard of commercial fish , while the male fish cannot meet the c

Method used

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Embodiment Construction

[0019] 1. Semi-smooth tongue sole fish roe and sea bass sperm

[0020] Using 20 semi-smooth tongue sole broodstock artificially bred by Haiyang Huanghai Fishery Co., Ltd., the female fish weighs 1-2kg / tail, the male fish weighs 150-300g / tail, and the ratio of male to female is 1:2. After reproductive regulation, it reaches sexual maturity and begins to give birth naturally. Unfertilized eggs of tongue sole were obtained by artificially squeezing the abdomen. In November 2002 and November 2003, perch sperm were cryopreserved with MPRS+20% DMSO, and the storage period was 3-4 years. After thawing in a 37°C water bath, the sperm viability remained at 80-90% under a microscope.

[0021] 2. UV Irradiation of Frozen / Thawed Perch Sperm

[0022] Draw 100 μL of the above perch sperm and place it in a pre-cooled petri dish with a diameter of 10 cm, add 900 μL of MPRS to dilute, shake well and put it on the bottom of the petri dish, use 1000 μj / cm 2 Ultraviolet meridians were irradiate...

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PUM

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Abstract

The invention relates to a method for using the frozen sperm of bass to induce the cultivation of sliding-tongue ovum thelykaryon, which comprises that: using the bass sperm frozen in the liquid nitrogen, to induce the thelykaryon cultivation of sliding-tongue ovum; defreezing the sperm in 37Deg.C water bath, taking 100muL sperm to dilute the MPRS for 10 times, to be arranged in the cultivate container at 6Deg. C; using 1000muj/cm2 ultraviolet to radiate for 0.2min, to inactivate the sperm; then using the inactive sperm to induce the fecundation with the ovum; in 5min of fecundation, using 2Deg. C water bath to shock the zygote, while the shock time is 20min; arranging the blast into the sea bath at 23Deg. C to be cultivated. The invention has low cost.

Description

technical field [0001] The technology belongs to the field of marine biotechnology, and is a breeding technology for cultivating all-female, fast-growing new breed of half-smooth tongue sole, which can be directly applied to the seawater fish farming industry. Background technique [0002] Semi-smooth tongue sole is a unique economic fish distributed in the Yellow Sea and Bohai Sea of ​​my country. After artificial domestication and breeding in recent years, it has been developed into a rare fish in seawater factory culture. The body weight is more than 4 times that of the males, and about 50% of the male fish in the breeding fry grow slowly, and it is difficult to reach the size of commercial fish, which increases the cost of breeding and production, and restricts the large-scale breeding of fry. Female fry have important practical significance for improving the economic benefits of farming and expanding the scale of farming. Gynogenesis is a type of parthenogenesis, which r...

Claims

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Application Information

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IPC IPC(8): A01K61/00A01K67/027A61D19/00A61K35/52
CPCY02A40/81
Inventor 田永胜陈松林邵长威刘本伟王清印庄志猛
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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