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Tobacco virus abrasive paper frictional inoculation method

A technology for tobacco virus and tobacco mosaic disease, applied in the directions of viruses/phages, biochemical equipment and methods, microorganisms, etc., can solve the problems of large inoculation virus amount error, difficult operation, complicated procedures, etc., and achieves easy access to materials and operation. Simple, effective results

Inactive Publication Date: 2012-04-11
GUIZHOU TOBACCO SCI INST
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AI Technical Summary

Problems solved by technology

The disadvantage is that the procedure is cumbersome, time-consuming and labor-intensive, and there is a large error in the amount of virus inoculated between plants, especially when a large area is inoculated and there are many materials.
The spray gun inoculation method is to add corundum to the juice of the diseased plant, and use the same injection pressure to inoculate the juice of the diseased plant containing a small amount of corundum on the inoculated plant at the same time. The disadvantage is that the operation is difficult. Control at the same time and the same pressure lacks certain operability; the spray gun equipment is more expensive, which makes the test cost higher
The diseased tissue is directly inoculated on the leaf surface, also known as the "dry grafting method". It is suitable for a small amount of inoculation or when the diseased tissue material is small and the virus is unstable. The amount of virus has a large error
[0003] After searching, there is no method of using sandpaper for inoculation, and there is no new inoculation method to apply for a patent

Method used

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  • Tobacco virus abrasive paper frictional inoculation method
  • Tobacco virus abrasive paper frictional inoculation method

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Embodiment 2004～20

[0019] EXAMPLES From 2004 to 2006, the field drug efficacy test of tobacco common mosaic disease was carried out in Dafang County, Guizhou Province every year, and there were about 3,500 flue-cured tobacco plants in more than 3 mu of land; from 2004 to 2006, every year in Jinsha County, Guizhou Province The China Tobacco Variety Breeding Base screens and identifies mosaic varieties. The test materials are about 5 mu of land and about 5,000 to 6,000 flue-cured tobacco plants.

[0020] 1. Take 20 g of leaves (leaf veins removed) of typical tobacco common mosaic disease symptoms, add a small amount of water and grind in a mortar, filter with gauze and squeeze out the juice, then use 1 to 5 L of distilled water to make up the volume to obtain the inoculum;

[0021] 2. Take 2 pieces of commercially available sandpaper, divide them into 8 small pieces of equal area, and fold them in half to form edges;

[0022] 3. Dip the inoculum with the edge of the sandpaper and gently draw it fr...

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Abstract

The invention discloses a abrasive paper rubbing method of tobacco virus disease, including steps: (1) bruising leaf tissue with typical symptom of tobacco virus disease in mortar, adding water, filtering by gauze and extruding juice, then constant volume by distilled water to obtain inoculation liquor, compouded when need; (2) dividing a abrasive paper into equal portions and folding as arris; (3) dipping arris of the abrasive paper with inoculation liquor, sliding from one end to the other end of the arris on inoculation leaf, finishing inoculation. The method gets material easily, has simple, quick operation with little inaccuracy, only has three steps, success ratio of inoculation is 100%. The method suits for tobacco virus disease infected by machinery juice such as TMV, CMV, PVY, PVX and TEV and so on, especially for selecting and identifying fastness of tobacco species and experiment of prevention and cure tobacco virus disease.

Description

technical field [0001] The invention relates to an inoculation method for tobacco diseases, in particular to a mechanical friction propagation inoculation method for tobacco virus diseases. technical background [0002] Inoculation test is the most important work in plant disease research. Inoculation test is a prerequisite for studying plant resistance to pathogens, disease cycle, disease prevalence, disease control, infection process and pathogenicity differentiation of pathogens. Tobacco Mosaic Virus (TMV), Tobacco Mosaic Virus (CMV) and Tobacco Potato Virus Y (PVY) are the main viral diseases of tobacco, and these tobacco virus diseases have mechanical At present, the inoculation methods of mechanical friction infection of tobacco virus disease mainly include the disease plant juice friction inoculation method, spray gun inoculation method, and direct inoculation method of diseased tissue. As far as the current tobacco virus disease inoculation methods are concerned, the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N7/00
Inventor 陈庆园郭华李继新商胜华
Owner GUIZHOU TOBACCO SCI INST
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