Separating purified new bisflavone compound from dragon's blood and preparation method thereof

A technology for separation and purification of compounds, applied in the field of pharmaceutical phytochemical extraction technology, can solve problems such as unseen

Inactive Publication Date: 2007-09-26
SHANGHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] On the isolation of a new biflavonoid compound 8-(3-(4-hydroxy-2-methoxyphenyl)-1-(4- The structure and preparation method of hydroxyphenyl)propyl)-2-(4-hydroxyphenyl)-7-methoxy-4H-benzopyran-4-one have not been reported by SciFinder; also There is no relevant report on the process of synthesizing the compound

Method used

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  • Separating purified new bisflavone compound from dragon's blood and preparation method thereof
  • Separating purified new bisflavone compound from dragon's blood and preparation method thereof
  • Separating purified new bisflavone compound from dragon's blood and preparation method thereof

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Effect test

Embodiment 1

[0026] Weigh 100g of 60-80 mesh dragon’s blood jerky powder, add 1800mL of petroleum ether for Soxhlet extraction, the temperature of Soxhlet extraction is 75°C, the extraction time is 12 hours, and the residual petroleum ether is volatilized from the raffinate. 94.5 g of petroleum ether raffinate A was obtained; the above raffinate A was subjected to solid-liquid extraction with 300 mL of acetone at room temperature, repeated 3 times, the extracts were combined, concentrated with a rotary evaporator, and dried at 40°C to obtain acetone extract B 84.4g; the above-mentioned extract B was subjected to solid-liquid extraction with 300mL ethyl acetate at room temperature, repeated 4 times, and the residual ethyl acetate in the raffinate was evaporated to dryness to obtain 36.2g of ethyl acetate raffinate C; After dissolving the above raffinate C with methanol, add an equal amount of silica gel and mix evenly, remove the methanol by volatilization, grind the solid sample into powder...

Embodiment 2

[0029] Weigh 100g of 60-80 mesh dragon's blood, add 2000mL of petroleum ether for Soxhlet extraction, the temperature of Soxhlet extraction is 70°C, the extraction time is 15 hours, and the residual petroleum ether is evaporated from the raffinate to obtain petroleum Ether raffinate A 97.2g; the above raffinate A was subjected to solid-liquid extraction with 250mL acetone at room temperature, repeated 5 times, the combined extracts were concentrated with a rotary evaporator, dried at 45°C to obtain 86.7g of acetone extract B Carry out solid-liquid extraction with 250mL ethyl acetate above-mentioned extract B at room temperature, repeat 5 times, the residual ethyl acetate in the raffinate is evaporated to dryness, obtain ethyl acetate raffinate C 38.2g; Take above-mentioned extract After dissolving the residue C with methanol, add an equal amount of silica gel and mix evenly, volatilize to remove methanol, grind the solid sample into powder in a mortar, pass through an 80-mesh s...

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Abstract

The invention discloses a making method of separated and purified new bis-flavonoid from dragon's blood, which is characterized by the following: adopting dragon's blood as raw material; extracting through ligarine, acetone, acetic ester solvent sequently; separating and recrystallizing the acetic ester solvent extract through silica gel column or rapid silica gel column to chromatograph; obtaining the product named 8-(3-(4-hydroxy-2-methoxybenzene)-1-(4-hydroxy benzene) propyl )-2-(4-hydroxy phenyl )-7-methoxy -4H-benzopyranyl -4-ketone to inhibit alpha-glucosidase acitivty and reduce blood sugar.

Description

technical field [0001] The invention relates to a novel biflavonoid compound isolated and purified from dragon's blood and a preparation method thereof, belonging to the technical field of medicinal phytochemical extraction technology. Background technique [0002] Flavonoids are a class of compounds with the core structure of 2-benzochromone, which are widely distributed in plants, and bisflavonoids are dimers formed by the polymerization of two molecular flavonoid derivatives. It is more concentrated in the gymnosperms except Pinaceae, especially in the Ginkgo class, and also exists in the Selaginella genus of the fern. Common natural biflavones are composed of two molecules of apigenin or its methyl ether derivatives. According to its combination mode, there are 3,8″-bispigenin type of ginkgo and isoginkgoin, and 8,8″ of papigenin -Di-apigenin type, diphenyl ether type of hinoki flavone, etc. Biflavonoids are a class of important biologically active substances, which ha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/30A61K31/352A61P3/10A61K36/896
Inventor 雍克岚吕敬慈卢大胜徐鲁荣陈旭
Owner SHANGHAI UNIV
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