Method for preparation of novel subunit vaccine for treating porcine pseudorabies

A subunit vaccine and porcine pseudorabies technology, applied in the direction of antiviral agents, virus antigen components, etc., can solve the problems of difficulty in control and elimination, inability to distinguish vaccine immune antibodies from virulent antibodies, and large local side effects of injection, etc., to achieve optimization Capsule extraction technology, the effect of solving poor immunogenicity and simplifying the production process

Inactive Publication Date: 2007-12-05
陈少莺
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Problems solved by technology

Because most of the adult pigs are latently infected, and become a potential source of infection due to long-term virus-carrying and shedding, it brings great difficulties to the control and elimination of the disease
At present, the effective prevention of PR in all countries in the world is mainly vaccine immunization. The existing porcine pseudorabies vaccines include oil emulsion inactivated vaccines, traditional attenuated vaccines and gene deletion vaccines. The shortcomings of these vaccines are: such as oil emulsion inactivated vaccines. The safety of the vaccine is good, but the immune effect is not ideal, the local side effects of the injection are large, and serological tests cannot distinguish the vaccine immune antibody from the virulent a

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  • Method for preparation of novel subunit vaccine for treating porcine pseudorabies
  • Method for preparation of novel subunit vaccine for treating porcine pseudorabies

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Embodiment Construction

[0007] The following test examples are used to further illustrate the present invention and the beneficial effects of the present invention (but not to limit the present invention).

[0008] The vaccine preparation method of the present invention is as follows: after the cytotoxicity of the PRV-FB strain is subjected to differential centrifugation, resuspended in PBS, the protein concentration of the virus antigen is adjusted, and Mega-10 split virus envelope protein is added, and extracted by centrifugation Envelope protein, adding appropriate ISCOM matrix, bagging dialysis is a new type of PRV subunit vaccine.

[0009] The PRV envelope protein subunit vaccine does not contain nucleic acid components, has good safety, and serological methods can distinguish naturally infected animals from vaccinated animals, but its immunogenicity is poor. For this reason, the inventors further adopted the following measures to improve its immune effect:

[0010] (1) The characteristics and culti...

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Abstract

The present invention discloses preparation process of new subunit vaccine of pig's pseudo rabies. The preparation process includes: differentially centrifuging pseudo rabies low virulent strain (PRV-FB strain), suspending the virus in PBS, regulating the protein concentration of the viral antigen, adding Mega-10 lytic virus cyst membrane protein, centrifugally extracting cyst membrane protein, adding proper amount of ISCOM matrix, bagging and dialyzing to obtain PRV type subunit vaccine. The PRV type subunit vaccine is safe and effective, and may be used in preventing and controlling pig's pseudo rabies.

Description

Technical field [0001] The invention relates to a method for preparing a novel subunit vaccine of porcine pseudorabies. Background technique [0002] Pseudorabies is a comorbid infectious disease of a variety of animals. Pigs are its natural host. After the infected pigs recover, they become invisible infection. It mainly causes abortion, stillbirth or mummification of pregnant sows. Newborn piglets show neurological symptoms, paralysis, exhaustion and death. The mortality rate is almost 100%, the incidence rate of weaned piglets is 20%-40%, and the mortality rate is 10%-20%. This disease has become the main cause of the current breeder reproductive disorder syndrome, and it is obviously increasing year by year. Because adult pigs are mostly invisible infections, and long-term poisoning and detoxification become a potential source of infection, which brings great difficulties to the control and elimination of the disease. At present, the effective prevention of PR in various coun...

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Application Information

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IPC IPC(8): A61K39/12A61P31/12
Inventor 陈少莺程晓霞俞伏松陈仕龙林天龙林梅
Owner 陈少莺
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