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Method for collecting high concentration milt of scallop

A high-concentration, semen technology, used in sensors, diagnosis, animal husbandry, etc., can solve the problems of high energy loss, low activation rate, unavoidable sperm activation, etc., to achieve the effect of simple collection method and easy operation

Inactive Publication Date: 2008-03-05
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Summary
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AI Technical Summary

Problems solved by technology

[0003] At present, most shellfish semen are collected by dissection and natural discharge. The former cannot guarantee the complete maturity of the obtained sperm, and the latter cannot prevent the sperm from being activated by seawater prematurely.
For example, the collection of abalone semen usually adopts a combination of drying in the shade and heating to induce ejaculation of abalone, and then centrifuges to concentrate the sperm produced in seawater [Liliana Salinas-Flores et al., Journal of shellfish, 2005, 24 (2): 415-420; Gwo Jin-Chywan et al., Theriogenology, 2002, 58: 1563-1578], but the spermatozoa obtained by centrifugal concentration have a relatively large energy loss due to long-term contact with seawater; Hughes (Cryobiology, 1973, 10: 342-344) and Zell et al. (Cryobiology, 1979, 16: 448-460) studies have shown that this seawater-sperm mixture is not ideal for oyster sperm freezing, and the method of dissecting gonad tissue to obtain semen is more successful, but it cannot guarantee the integrity of the obtained sperm. Mature, and many other tissue cells remain in the semen
Through cryopreservation and insemination of scallop sperm, a method for collecting scallop semen with high concentration, high maturity rate and low activation rate has been found, but there is no relevant report yet

Method used

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Embodiment Construction

[0014] The present invention is described in detail below by specific embodiment:

[0015] The specific operation process of the best embodiment:

[0016] From March to June 2005, with the shell height as the standard, select fast-growing, healthy and lively scallops for temporary breeding to establish a high-quality broodstock group; select male individuals with mature gonads from this group, and use 200 μL of yellow suction tips to The renal tube is punctured near the end of the renal foramen, and the semen is aspirated and collected. The sperm motility monitoring, cryopreservation at ultra-low temperature and fertilization test prove that the semen obtained by this method has the characteristics of high concentration, high maturity and low activation rate.

[0017] The specific operations to complete the above operation process are as follows:

[0018] The steps include: 1. Cultivation of high-quality broodstock; 2. Structural observation of reproductive system and excret...

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Abstract

The present invention relates to a method for collecting high-concentration seminal fluid of scallop. Said method includes the following several procedures; incubation of high-quality parent scallop, preparation of suction head for sucking seminal fluid, collection of high-concentration seminal fluid and detecting vitality of collected spermatozoa. Said invention also provides the concrete requirements and steps of above-mentioned every procedure.

Description

Technical field: [0001] The invention belongs to the technical field of marine biology, belongs to the technological improvement in the genetic breeding research of shellfish, and relates to a technical method for collecting scallop semen with high concentration, high maturity rate and low activation rate in the peak breeding period of scallops. Background technique: [0002] Semen collection generally refers to artificial insemination research or technical methods to obtain semen as the research object in reproductive disorder research. [0003] At present, most shellfish semen are collected by dissection and natural discharge. The former cannot guarantee the complete maturity of the obtained sperm, and the latter cannot prevent the sperm from being activated by seawater prematurely. For example, the collection of abalone semen usually adopts a combination of drying in the shade and heating to induce ejaculation of abalone, and then centrifuges to concentrate the sperm prod...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61D19/00A01K61/00G01N33/00A61B5/00
CPCY02A40/81
Inventor 杨爱国周丽青刘志鸿杨培民
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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