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Sugarcane detoxication tissue culturing and fast propagating method

A technology of tissue culture and rapid propagation of sugarcane, applied in horticultural methods, botanical equipment and methods, cultivation, etc., can solve problems such as pollution, low survival rate, inability to completely remove viruses, etc., and achieve recovery of yield and quality, and detoxification Simple and effective detoxification effect

Inactive Publication Date: 2011-03-30
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the problem with this shoot apical meristem detoxification method is that it is difficult to cut out very small shoot apical meristems below 0.2mm, and it needs to be operated under a microscope; The lower the rate, the larger the cut shoot tip tissue, the virus cannot be completely removed, and it is easy to cause pollution; therefore, there is an urgent need for a better method in production to meet the needs of sugarcane virus-free tissue culture and rapid propagation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 (The top buds of sugarcane are explants)

[0029] 1) Selection and sterilization of explants: from June to November, take the apical buds of sugarcane, soak in 75% alcohol for 0.5 to 1.0 minutes, then sterilize with 0.1% mercury solution for 10 minutes, and finally use sterile water Wash 3~5 times as explants for detoxification tissue culture;

[0030] 2) Minimal medium: use MS minimal medium, sugar 20g / L, agar 9g / L, pH 5.6;

[0031] 3) Callus induction culture: Under aseptic conditions, extract 2~3mm shoot tip tissue from the explant after sterilization in step (1), and inoculate it in MS+2,4-D1mg / L+NAA0.1mg / L callus induction medium, to induce the formation of callus;

[0032] 4) Differentiation culture: transplant the callus induced in step (3) to MS+6-BA2.0mg / L+NAA0.1mg / L differentiation medium to induce clump buds;

[0033] 5) Proliferation culture: cut the differentiated clump buds in step (4) into individual plants and inoculate them on the proliferation mediu...

Embodiment 2

[0037] Example 2: (Take the axillary bud of sugarcane as explant 1)

[0038] 1) Selection and sterilization of explants: In November, take the axillary buds of sugarcane, soak them in 75% alcohol for 0.5~1.0min, then sterilize them with 0.1% mercury solution for 15min, and finally rinse with sterile water for 3~5 Second, as an explant for detoxification tissue culture;

[0039] 2) Basic medium: use MS basic medium, sugar 25g / L, agar 8g / L, pH 5.7;

[0040] 3) Callus induction culture: under aseptic conditions, extract 2~3mm shoot tip tissue from the explant after sterilization in step (1), and inoculate it in MS+2,4-D2mg / L+NAA0.2mg / L callus induction medium, induce the formation of callus;

[0041] 4) Differentiation culture: transplant the callus induced in step (3) to MS+6-BA3.0mg / L+NAA0.3mg / L differentiation medium to induce clump buds;

[0042] 5) Proliferation culture: cut the differentiated clump buds in step (4) into individual plants, inoculate them on the proliferation medium...

Embodiment 3

[0046] Example 3: (Take the axillary buds of sugarcane as explant 2)

[0047] 1) Selection and sterilization of explants: In November, take the axillary buds of sugarcane, soak them in 75% alcohol for 0.5-1.0 minutes, then sterilize them with 0.1% mercury solution for 10 minutes, and finally rinse with sterile water for 3-5 Second, as an explant for detoxification tissue culture;

[0048] 2) Minimal medium: use MS minimal medium, sugar 30g / L, agar 7g / L, pH 5.8;

[0049] 3) Callus induction culture: Under aseptic conditions, extract 2~3mm stem apex tissue from the explant after sterilization in step (1), and inoculate it in MS+2,4-D1.5mg / L+NAA0 .15mg / L callus induction medium, induce the formation of callus;

[0050] 4) Differentiation culture: transplant the callus induced in step (3) to MS+6-BA2.5mg / L+NAA0.15mg / L differentiation medium to induce clump buds;

[0051] 5) Proliferation culture: cut the differentiated clump buds in step (4) into individual plants and inoculate them on th...

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Abstract

The present invention relates to a method for virus-free tissue culture and rapid propagation of sugarcane belonging to the technical field of plant propagation. The method comprises the following steps that the terminal buds or auxiliary buds of the sugar cane is taken as the explants and the virus-free tissue culture seedlings are propagated in mass after the callus tissue inducement and culture, plant differentiation culture, proliferation culture, radication culture and virus test. The present invention is characterized in that the explants adopted are large and the operation is easy; theinoculation survival rate is high; the detoxification is simple, convenient, fast and thorough; the propagation coefficient per month is 5 to 10 times and the speed is fast which is fit for breeding in factory and commercial production.

Description

Technical field [0001] The invention relates to the technical field of plant detoxification tissue culture and rapid propagation, in particular to a method for sugarcane detoxification tissue culture and rapid propagation. Background technique [0002] Sugarcane mosaic disease has been widespread in Zhejiang, Fujian and other provinces for many years. New leaves show light yellow mottled or slight chlorotic streaks, and old leaves show long chlorotic streaks or mosaic leaves. The incidence is very high, causing sugar and yield. Decline (about 18%), the species also decline, affecting quality. [0003] There are many methods to obtain virus-free plants, such as heat treatment detoxification, shoot tip culture detoxification, shoot tip micrografting and combination methods. Among them, the better method commonly used is the shoot tip culture detoxification method. The specific steps are: cut the shoot tip (meristem below 0.2mm) through the plant apical meristem, induce the productio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00C12N5/04A01G31/00
Inventor 徐刚汪一婷牟豪杰吕永平陈剑平
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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