Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Trigone-leaf dioscorea opposita saponin liposome, method for preparing the same and use of the same

A technology of dioscin and trigonum saponin, which is applied in the field of medicine, can solve the problems that there is no preparation of dioscin trigonum saponin, and achieve the effect of treating cardiovascular diseases, uniform particle size and shape, and good stability

Inactive Publication Date: 2008-04-02
WEST CHINA HOSPITAL SICHUAN UNIV
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no relevant report on making diosgenin from trigonum leaf into liposomes

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1 Preparation of Diosgenin Liposomes by High Pressure Homogenization

[0016] Get lecithin (Chengdu Kelong Chemical Reagent Co., Ltd.) 2.5g, cholesterol (Chengdu Kelong Chemical Reagent Co., Ltd.) 0.5g, add 15ml dioscin phosphate buffer (containing dioscin 0.5g) and heat up to 80°C; add this high-temperature liquid phase into the double myristyl phosphatidylglycerol solution at the same temperature, mix quickly for 1min (8500rpm), and then mix with 500bar high pressure for 3 times; finally cool down and solidify. The encapsulation efficiency was 65%.

Embodiment 2

[0017] Example 2 Preparation of dioscin liposomes by thin film method

[0018] Dissolve 1.25 g of lecithin (Chengdu Kelong Chemical Reagent Co., Ltd.) and 0.3 g of cholesterol (Chengdu Kelong Chemical Reagent Co., Ltd.) in 60 ml of ether, and use a 250 ml eggplant-shaped bottle to evaporate under reduced pressure on a rotary evaporator to remove ether. The lipid forms a thin film on the wall of the Erlenmeyer flask, and then 15 ml of dioscin phosphate buffer (containing 0.3 g of dioscin) is added to rotate and hydrate to obtain dioscin liposomes. The encapsulation efficiency is 70%.

Embodiment 3

[0019] Example 3 Preparation of Dioscin Liposomes by Reverse Evaporation

[0020] Dissolve 2.5g of lecithin and 1g of cholesterol in a 250ml Erlenmeyer flask with 120ml of ether, then add 20ml of 8% mannitol aqueous solution of diosgenin (containing 0.4g of diosgenin), and ultrasonically make it into a homogeneous single-phase system , and then evaporated under reduced pressure to remove ether to a gel state, and then continued to evaporate under reduced pressure for 10 minutes to obtain diosgenin liposomes. The encapsulation efficiency is 50%.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention belongs to the field of medicine and relates to a triangle leaf yam saponin liposome and the preparing method and the purpose. The liposome of the invention is prepared according to a weight percentage as follows: 1 portion of triangle leaf yam saponin and 0.5 portion to 15 portion of lipid. The lipid comprises at least one of fatty acid, triglyceride, waxy type and steroid. The present invention can be prepared with anyone method of high-pressure homogenization, thin-film method, reverse evaporation method, freezing-drying method and melting method. Particle size morphology of the prepared liposome is even and is 1 Mu m to 6 Mu m. The encapsulation is high. The stability is good. The present invention is consistent with Pharmacopoeia requirements and has excellent treatment effect on cardiovascular disease.

Description

technical field [0001] The invention relates to diosgenin liposome and its preparation method and application, belonging to the field of medicine. Background technique [0002] With the development and changes of people's social life, cardiovascular disease is increasingly endangering the life expectancy and quality of life of human beings. It is one of the diseases with the highest morbidity and mortality in the world. According to relevant statistics, the incidence of some cardiovascular diseases in my country: coronary heart disease is 10%, acute myocardial infarction is 15.4 / 100,000, coupled with the acceleration of population aging, and the main manifestations of stroke and myocardial infarction are fatal The incidence of cardiovascular disease with disability and fatal consequences has risen sharply, and it is showing a younger trend. At present, there are more than 13 million patients with cardiovascular diseases in my country, and the incidence rate is increasing by ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/127A61K9/12A61K9/72A61K47/10A61K47/12A61K47/24A61K47/44A61K31/7048A61K36/8945A61P9/10A61K47/28
Inventor 黄文王旺魏蒙冀承杰何扬侯晓敏李丹程惊秋
Owner WEST CHINA HOSPITAL SICHUAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com