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Producing strain for beta-dextranase

A glucanase and bacteria-producing technology, applied in the field of microorganisms, can solve problems such as poor thermal stability of enzymes, and achieve the effects of good acid resistance, good research value, and good heat resistance.

Inactive Publication Date: 2008-04-09
YUNNAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a kind of β-glucanase producing bacteria that can effectively solve the problem of poor thermostability of enzymes used in β-glucanase industry at the present stage, and have good heat resistance

Method used

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  • Producing strain for beta-dextranase
  • Producing strain for beta-dextranase

Examples

Experimental program
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Effect test

Embodiment

[0022] 1. Isolate the strain W9 from (Anning) wheat soil, directly coat the primary screening medium plate and culture at 50°C for 3-5 days, observe the growth of the transparent circle, and select the strain with a larger H / C ratio (H / C is the ratio of the diameter H of the transparent circle to the diameter C of the colony), and the pure culture β-glucanase strain W-9 was isolated. The screening medium used: β-glucan 2.5, Congo red solution 8mL, agar 2.0, pH natural.

[0023] 2. Identification of strains

[0024] According to morphological characteristics, cultural characteristics, physiological and biochemical measurements, and 16S rDNA sequence analysis, systematic classification research was carried out.

[0025] Strain W-9 is a Gram-positive bacillus with mesospores. On the LB agar plate, the colonies are white, with wavy edges and irregular shapes, and the surface is wrinkled and opaque. The growth temperature range of W9 is 40-60°C, it can hydrolyze starch, casein, ...

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Abstract

The invention relates to a producing strain of Beta mannanase. The classification and nomenclature of the producing strain of Beta mannanase is Bacillus subtilis with accession number of CGMCC No.2077. The most suitable pH and temperature for the glucanase produced by the producing strain of Beta mannanase of the invention are respectively 6.0 and 70 DEG C, enzyme activity changes little under the temperature of 50-70 DEG C and the rest enzyme activity is 20 percent after the glucanase being treated for 5 hours under the temperature of 60 DEG C. The invention has fairly good heat resistance and research value.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a β-glucanase producing bacterium. Background technique [0002] β-glucanase (β-glucanase) is a kind of structural non-starch polysaccharide (NSP), which is the sugar with the highest content of naturally synthesized polysaccharides. A glucose polymer composed of dextrose (D-type) linearly linked by mixed β-(1,3), β-(1,4) glycosidic linkages randomly arranged. The β-glucan contained in cereals has a unique molecular structure, which endows it with unique properties: it is soluble in water in the form of high molecular weight, and the viscosity of the formed solution is very high, which brings a lot of inconvenience to industrial production. For example, high molecular weight barley β-glucan can increase the viscosity of wort and beer, making it difficult to filter the wort, reducing the yield, and affecting the stability and quality of beer; using barley and other grains ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N15/31C12N9/42C12R1/125
Inventor 黄遵锡王丽丽
Owner YUNNAN NORMAL UNIV
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