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Method for cultivating drought-resistant and /or growth-delaying plant in hostile environment

A plant and adversity technology, applied in botany equipment and methods, biochemical equipment and methods, plant peptides, etc., can solve problems such as unclear functions and no reports

Inactive Publication Date: 2008-04-09
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the use of genetic methods, such as gene knockout technology, to study the specific CDPKs involved in ABA-regulated seed maturation and germination, seedling growth, stomatal movement, and plant stress tolerance has rarely been reported.
Until recently, the role of CPK3 and CPK6 in stomatal motility was investigated through T-DNA knockout mutants of CPK3 and CPK6, and other physiological aspects of their functions were obscure or unreported

Method used

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  • Method for cultivating drought-resistant and /or growth-delaying plant in hostile environment
  • Method for cultivating drought-resistant and /or growth-delaying plant in hostile environment
  • Method for cultivating drought-resistant and /or growth-delaying plant in hostile environment

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1, cultivating drought-resistant and delayed-growing plants in adversity

[0037] 1. Increase the expression of CPK11 in the target plant

[0038] 1. Construction of CPK11 overexpression vector pCAMBIA-35S-CPK11

[0039] The open reading frame of CPK11 was amplified by PCR using the double-stranded cDNA obtained by reverse transcription of the total RNA of Arabidopsis thaliana (Columbia, Col) as a template. The sequence of the upstream primer p1 used for PCR amplification was 5'-CATGCCATGGCGATGGAGACGAAGCCAAACCCTAG-3', and the sequence of the downstream primer p2 was 5'-GAAGATCTTCAGTCATCAGATTTTTCACCA-3'. The PCR product was sequenced according to conventional methods, and the sequencing results showed that the nucleotide sequence of the PCR product was the deoxyribonucleotide from the 76th to the 1563rd position of the 5' end of GenBank Accession Number AY050981. The PCR product and pCAMBIA-1304 ( http: / / www.cambia.org / daisy / cambia / materials / vectors / 585.h ...

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Abstract

The invention discloses a plant cultivating method that the plant has abilities of anti-drought and / or postponing their growth in adverse circumstances. The method enhances the CPK11 expression level in the targeted plant and gets the plant with the abilities of anti-drought and / or postponing their growth in adverse circumstances. The method has important application value in crop genetic improvement and agriculture development, in particular to the practical value of enhancing the anti-drought ability of the grain crops and the economic crops.

Description

technical field [0001] The present invention relates to a method of breeding drought-resistant and / or delayed-growing plants under stress. Background technique [0002] The plant hormone abscisic acid (ABA) is involved in the regulation of many aspects of plant growth and development, including seed maturation and germination, seedling growth, flowering, and stomatal movement; it is also a key plant response to various abiotic stresses such as drought, high salinity, and low temperature hormone. However, the ABA signal transduction pathway is still unclear, and further identification and research of the signaling components are needed. [0003] Calcium ions play an important role as a second messenger in ABA signal transduction. Calcium-dependent protein kinase (CDPK) is a calcium sensor that has been studied more in plants. CDPKs is a multi-gene family, and CPK4, CPK11, CPK3, CPK6, CPK32, CPK10 and CPK30 are all members of the CDPK family, and there are functional redund...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/09C12N15/82C12N15/29C07K14/415
Inventor 张大鹏祝赛勇于祥春王晓静王小芳
Owner CHINA AGRI UNIV
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