Promoter for introducing extracellular substance into mammalian ovum and introduction method

A technology of mammals and accelerators, which is applied in the field of introduction accelerators and introduction of extracellular substances into mammalian eggs, which can solve the problems of adverse effects on embryos and low success rate

CN101160398AInactive Publication Date: 2008-04-09JAPAN SCI & TECH CORP
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2008-04-09
Estimated Expiration
Not applicable · inactive patent

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Abstract

It is intended to provide a method, upon introducing an extracellular substance such as a sperm, a cell nucleus, a nucleic acid or a protein into a mammalian ovum, for introducing the extracellular substance into the mammalian ovum, which is safe for the mammalian ovum, enables efficient introduction, and is technically easy. By treating the mammalian ovum with a tubulin polymerization inhibitor such as vinblastine, membrane fusion between the ovum and the sperm is promoted and without making a hole in the cell membrane, the sperm and the ovum are fused and fertilization can be achieved. The method of the invention can be applied not only to the case where a sperm is introduced into a mammalian ovum, but also to a case where an extracellular substance such as a cell nucleus, a nucleic acid or a protein is introduced into a mammalian ovum.
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Description

technical field

[0001] The present invention relates to an extracellular substance introduction promoter for mammalian eggs containing a tubulin polymerization inhibitor as an active ingredient, and a method for introducing extracellular substances into mammalian eggs using the introduction promoter. More specifically, using Extracellular substance introduction promoter for mammalian eggs containing tubulin polymerization inhibitor as an active ingredient, method for introducing extracellular substances such as sperm, nucleus, nucleic acid, and protein into mammalian eggs, and breastfeeding for the method Animal egg import promoter for extracellular substances. One of the characteristics of the present invention is to provide an attempt to improve the fertilization ability of mammalian eggs by treating mammalian eggs with an extracellular substance introduction promoter containing a tubulin polymerization inhibitor as an active ingredient, and to promote the transfer of sperm ...

Examples

Embodiment 1

[0049] [Fertilization efficiency increased by vinblastine treatment]

[0050] In order to study the effect of a tubulin polymerization inhibitor on the fertilization of mouse unfertilized eggs, vinblastine was used as a tubulin polymerization inhibitor, and mouse unfertilized eggs were treated with vinblastine to investigate their fertilization ability.

[0051] (materials and experimental conditions)

[0052] 1. Culture composition (g / L) (TYH medium)

[0053] NaCl 6.976

[0054] KCL 0.356

[0055] CaCl 2 0.190

[0056] K H 2 PO 4 0.162

[0057] MgSO 4 ·7H 2 O 2.930

[0058] NaHCO 3 2.106

[0059] Glucose 1.000

[0060] Sodium pyruvate 0.550

[0061] Penicillin G 0.750

[0062] Streptomycin 0.050

[0063] Bovine Serum Albumin F-V 4.000

[0064] 1% phenol red 1.0ml

[0065] 2. Acidic Tyrode solution (g / L)

[0066] NaCl 8.00

[0067] KCl 0.20

[0068] CaCl 2 2H 2 O 0.24

[0069] MgCl 2 ·6H 2 O 0.10

[0070] Glucose 1.00 ...