A composition containing ascarin and its application
A technology of ascarin and composition, which is applied in the field of compositions containing ascarin, can solve problems such as complex components, improve fertilization ability, improve sperm acrosome reaction and motility, and reduce the risk of fertilization failure Effect
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Embodiment 1
[0033] Example 1 F10-Evaluation of the effect of the composition containing asthenoside on the improvement of sperm motility in severe asthenozoospermia
[0034] Sample Preparation
[0035] Sperm samples from patients with severe asthenozoospermia were selected for the experiment. Semen ejaculation by masturbation, using a specific sterile container to collect sperm samples after 2-7 days of abstinence. The samples were kept in a water bath at 22-33°C, and the liquefied semen was evaluated for sperm parameters (volume, sperm count, percentage of motility and motility characteristics) using the computer-assisted semen analyzer CASA according to the WHO1999 evaluation criteria. Asthenozoospermia is defined as grade A sperm count <25% or A+B <50%, (World Health Organization, 1999), while all other parameters are normal. Specimens containing white blood cells or somatic cell contamination were excluded based on morphological observations. The motility parameters of severe asthe...
Embodiment 2
[0045] Example 2 F10-Evaluation of the effect of the composition containing asthenosperm on improving the motility of sperm in moderate to mild asthenozoospermia
[0046] 1. Sample preparation
[0047] Sperm samples from patients with moderate to mild asthenozoospermia were selected for the experiment. Semen ejaculation by masturbation, using a specific sterile container to collect sperm samples after 2-7 days of abstinence. The samples were kept in a water bath at 22-33°C, and the liquefied semen was evaluated for sperm parameters (volume, sperm count, percentage of motility and motility characteristics) using the computer-assisted semen analyzer CASA according to the WHO1999 evaluation criteria. Asthenozoospermia is defined as grade A sperm count <25% or A+B <50%, (World Health Organization, 1999), while all other parameters are normal. Specimens containing white blood cells or somatic cell contamination were excluded based on morphological observations. The motility para...
Embodiment 3
[0056] Example 3 F10-Evaluation of the effect of the composition containing ascarin on the sperm motility of normal sperm
[0057] 1. Sample preparation
[0058] Normal sperm samples donated by healthy men were selected for the experiment. Semen ejaculation by masturbation, using a specific sterile container to collect sperm samples after 2-7 days of abstinence. The samples were kept in a water bath at 22-33°C, and the liquefied semen was evaluated for sperm parameters (volume, sperm count, percentage of motility and motility characteristics) using the computer-assisted semen analyzer CASA according to the WHO1999 evaluation criteria. Asthenozoospermia is defined as grade A sperm count <25% or A+B <50%, (World Health Organization, 1999), while all other parameters are normal. Specimens containing white blood cells or somatic cell contamination were excluded based on morphological observations. The motility parameters of severe asthenozoospermia sperm are (a+b) grade <25%, o...
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