Manufacturing method of biology enzymic electrode used for detecting pesticide residue

Abstract

The invention relates to a production method of a biological enzyme electrode used for the detection of pesticide residues, which pertains to the biochemistry electrodes, the production method adopts a combination method of nanoparticles and layer-by-layer self-assembly technology, the method has the steps that: a rod-shaped electrode carrier is made by using organic insulation materials, the substrate conductive materials are coated on a cross section of the lower end of the electrode carrier to form an electrode or the similar electrode is bought directly, then the outer surface of the conductive materials of the electrode uses the nanoparticles to decorate the electrode to form a nanoparticle layer, an anti-interference layer is prepared by the layer-by-layer self-assembly, an electrostatic adsorptive enzyme layer is prepared by the layer-by-layer self-assembly, so as to obtain a reaction layer which comprises the nanoparticle layer, the anti-interference layer and the electrostatic adsorptive enzyme layer, and the biological enzyme electrode used for detection of the pesticide residues is produced further. The production method of the invention is simple; a biosensor which is assembled by the biological enzyme electrode which is produced by the method has high sensitivity, stable performance and strong anti-interference capacity, so the invention is applicable to the actual application of the industrialization of the biological sensors for the detection of the pesticide residues.

Description

technical field [0001] The technical scheme of the invention relates to a biochemical electrode, in particular, a method for making a biological enzyme electrode for detecting pesticide residues. Background technique [0002] In recent years, the problem of excessive pesticide residues in agricultural products has attracted widespread attention. Biosensors for detecting pesticide residues have also been continuously developed and utilized. [0003] Mulchandani et al. (Flow injection amperometric enzyme biosensor for direct determination of organphosphate nerve agents, Environ.Sci.Technol.2001, (35)) combined flow injection technology to develop a flow injection amperometric organophosphate hydrolase biosensor (FIAB), which detects organic Phosphorus hydrolysis product p-nitrophenol, to determine the concentration of organic phosphorus. Jeanty et al. (Detection of paraoxonby continuous flow system based enzyme sensor, Biosensors and Bioelectronics.1998, (13)) used a flow in...

AI Technical Summary

Problems solved by technology

It directly mixes the enzyme and carbon nanotubes and drops them on the working electrode, and then detects the substrate, which inevitably has the following disadvantages: (1) the amount of enzyme cannot be well controlled (2) the detection process is easily affected by interfering substances , that is, the anti-interference ability is not strong, (3) the enzyme immobilization is not firm enough, resulting in poor electrode stability and poor repeatability

[0004] In conclusion, in the existing methods of using biosensors to test pesticide residues, the sensitivity, stability and anti-interference ability of biosensors still need to be further improved.

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