Tiny RNA detecting probe used for stomach organization and detection method thereof
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
An RNA probe, a tiny technology, applied in the field of microRNA, which can solve problems that do not involve gastric tissue
Inactive Publication Date: 2008-05-21
NINGBO UNIV
View PDF0 Cites 17 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
[0005] my country is an area with a high incidence of gastric cancer, and none of the existing technologies has involved which highly expressed microRNAs are related to the canceration of gastric tissue
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0020] A microRNA probe for gastric tissue. The sequence of the probe consists of a chemically modified oligonucleotide coding segment and an extension arm segment. The oligonucleotide coding segment includes the following microRNA: hsa-miR -186 * , hsa-miR-20b, hsa-miR-18a, hsa-miR-181d, hsa-miR-424, hsa-miR-18b, hsa-miR-17, hsa-miR-20a, hsa-miR-106a, hsa - miR-374a, hsa-miR-224, hsa-miR-181b, hsa-miR-25, hsa-miR-99a, hsa-miR-148b, hsa-miR-421, hsa-miR-199a-3p, hsa -miR-214 and hsa-miR-92a, hsa-miR-574-5p, the oligonucleotide coding segment can be complementary to the target microRNA, and the extension arm segment can make the coding segment connected to it have a certain distance from the film base Distance, reducing hybridization steric hindrance, the Tm value of probe hybridization is balanced by chemical modification, and the probe is synthesized in situ by photosensitive reagent into a microfluidic chip.
Embodiment 2
[0022] A microRNA probe for gastric tissue, which is basically the same as in Example 1, except that the oligonucleotide coding fragment also includes the following microRNAs: hsa-miR-93, hsa-miR-92b, hsa - miR-100, hsa-miR-638, hsa-miR-106b, hsa-miR-923, hsa-miR-125a-5p, hsa-miR-23a, hsa-miR-199a-5p, hsa-miR-663 , hsa-miR-99b, hsa-miR-125b, hsa-miR-23b, hsa-miR-181a and hsa-miR-10b.
Embodiment 3
[0024] A microRNA probe for gastric tissue, which is basically the same as in Example 1, except that the oligonucleotide coding fragment also includes the following microRNAs: hsa-miR-21, hsa-miR-27a, hsa -miR-183, hsa-miR-483-5p, hsa-miR-361-5p, hsa-miR-19b, hsa-let-7i, hsa-miR-149 * , hsa-miR-191, hsa-miR-146b-5p, hsa-miR-27b, hsa-miR-451, hsa-miR-24, hsa-miR-151-5p and hsa-miR-26a.
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Login to view more
Abstract
The invention discloses a tiny RNA probe which is used for stomach tissue and a detection method hereof; the sequence of the probe consists of an oligonucleotide coding segment and an extended arm segment which are chemically modified; the oligonucleotide coding segment comprises the following tiny RNAs of hsa-miR-186, hsa-miR-20b, hsa-miR-18a, hsa-miR-181d, hsa-miR-424, hsa-miR-18b, hsa-miR-17, hsa-miR-20a, hsa-miR-106a, hsa-miR-374a, hsa-miR-224, hsa-miR-181, hsa-miR-25, hsa-miR-99a, hsa-miR-148b, hsa-miR-421, hsa-miR-199a-3p, hsa-miR-214, hsa-miR-92a and hsa-miR-574-5p; crossbreeding detection of the tiny RNA of the stomach tissue is implemented and the high-expression tiny RNA when the stomach tissue is cancerized can be detected; in this way, the high-expression tiny RNA is taken as a diagnosis mark of the cancerization of the stomach tissue; the tiny RNAs can be further designed to be the target points which orient the stomach cancer drug; therefore, the invention provides beneficial basis for judging stomach cancer and orienting the treatment.
Description
technical field [0001] The invention relates to microRNA, in particular to a microRNA probe for detecting gastric tissue and a detection method thereof. Background technique [0002] MicroRNA is a kind of single-stranded small molecule RNA with a length of 19-24nt, which is relatively conservative in evolution and does not encode protein. It is a newly discovered gene regulator in recent years; It plays an important regulatory role in the cleavage or translation inhibition. [0003] MicroRNAs play a pivotal role in the normal development of organisms. About 250 microRNAs have been found in the human genome. Some microRNAs have obvious abnormal expressions in the physiological abnormal variation of different tissue cells, specifically in The occurrence of tumors in different tissues is closely related to the abnormal expression and function of certain microRNAs. About 50% of the annotated microRNAs are located on the genome as fragile sites associated with tumors, so differe...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more 
Login to view more