Pig replication and respiration syndrome virus attenuated vaccine strain and uses thereof

A technology for respiratory syndrome and attenuated vaccines, applied in the field of porcine reproductive and respiratory syndrome virus attenuated vaccine strains and attenuated vaccine strains, can solve the problems of poor vaccine pertinence, unsatisfactory immune effect, antigenic variation, etc., and achieve good safety Effect

Active Publication Date: 2008-07-16
HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although commercialized inactivated vaccines and attenuated vaccines have been developed abroad, due to the characteristics of antigenic variation and drift of the virus, foreign vaccines are relatively poorly targeted and often have unsatisfactory immune effects, and there have been reports abroad , an outbreak of the disease was caused by the use of attenuated PRRS vaccine

Method used

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  • Pig replication and respiration syndrome virus attenuated vaccine strain and uses thereof
  • Pig replication and respiration syndrome virus attenuated vaccine strain and uses thereof
  • Pig replication and respiration syndrome virus attenuated vaccine strain and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0021] 1.1 The pigs used in the test were purchased from PRRS seronegative pig farms in non-epidemic areas.

[0022] 1.2 Virus cell culture and cloning

[0023] 1.2.1 Cell culture Marc-145 cell line was cultured with DMEM cell culture medium containing 4% inactivated calf serum, 100 U / ml penicillin, 80 μg / ml streptomycin, and NaHCO 3 Adjust the pH value to about 7.2, and filter to sterilize. Use this cell culture solution to resuscitate or subculture the cell line Marc-145 into a sterilized small flat bottle with a capacity of 100ml, and use it to inoculate the virus after it grows into a good cell monolayer.

[0024] 1.2.2 Cloning of the virus The PRRSV CH-1a strain isolated from China was passaged on Marc-145 cells, and virus clones were carried out at the 30th, 50th, 70th, 90th, 110th, and 130th generations, except for the 30th generation A 2mm plaque was selected for seeding clones, and a 1mm plaque was selected for subsequent clones. The 30th and 50th generation seed p...

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Abstract

The invention discloses a porcine reproductive and respiratory syndrome virus attenuated vaccine strain, and the microorganism collection number is CGMCC NO.1883. The attenuated vaccine strain of the invention has stable genetic variation and good safety, which is safe and non-pathogenic for pregnant sows, piglets and the pigs at various ages. The immunized piglets after using the attenuated vaccine strain of the invention for immunization can resist the attack of the strong virus of the porcine reproductive and respiratory syndrome virus.

Description

technical field [0001] The invention relates to an attenuated vaccine strain, in particular to an attenuated vaccine strain of porcine reproductive and respiratory syndrome virus and its application, belonging to the field of biotechnology. Background technique [0002] Porcine Reproductive and Respiratory Syndrome (Porcine Reproductive and Respiratory Syndrome, PRRS) is caused by PRRSV, characterized by reproductive disorders, respiratory symptoms, and highly contagious viral disease. The disease was first discovered in the United States in 1987. Wensvoort et al. isolated and identified the pathogen of the disease in 1991. PRRSV belongs to the Arterivirus genus of the family Arteriviridae (Arteriviridae), and is a positive-sense single-stranded RNA virus with an envelope. Since PRRSV has the characteristics of antigenic drift and variation, and its main target cells are alveolar macrophages (PAM), which have important immune functions, PRRSV infection is often accompanied b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/00A61K39/12A61P31/14
Inventor 蔡雪辉王洪峰刘永刚郭宝清柴文君
Owner HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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