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Embedded immobilization microbial fertilizer and its preparing method

A technology for immobilizing microorganisms and fertilizers, applied to the direction of fixing on/in organic carriers, organic fertilizers, etc., can solve problems such as poor immobilization effect, insufficient particle hardness, and reduce the concentration of bacteria in embedded particles, so as to improve Sustainable utilization, simple production process, increased yield and improved crop quality

Inactive Publication Date: 2008-08-13
INST OF SOIL & FERTILIZER SHANDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the field of agriculture, such as Chinese patent document CN1490279A (patent No. 03125307.5), Hu Xiaojia, etc. use a single sodium alginate as an embedding agent and active carbon as an adsorbent to fix three kinds of bacteria for the production of microbial fertilizers, but use a single Sodium alginate as an embedding material often has poor fixation effect, and the hardness of the particles is not enough. At the same time, the adsorption of bacteria by activated carbon virtually reduces the concentration of bacteria in the embedded particles.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] An embedded and immobilized microbial fertilizer has the following raw material components: 20 kg of embedded bacteria and 80 kg of embedding agent.

[0033] Preparation method, concrete steps are as follows:

[0034] 1. Take the Bacillus megaterium bacterial liquid in the logarithmic growth phase, centrifuge it on a centrifuge at 5000r / min for 20min, and weigh 20kg of the precipitate as the embedded bacteria;

[0035] 2. Dissolve sodium alginate in water at a temperature of 70-90°C to make an aqueous solution with a concentration of 8%wt; take gelatin and dissolve it in water at a temperature of 70-90°C to make an aqueous solution with a concentration of 8%wt; Mix the above two aqueous solutions in a volume ratio of 1:1 to obtain an embedding agent solution;

[0036] 3. Take CaCl 2 Dissolve in water to make 2.2% CaCl by weight 2 aqueous solution;

[0037] 4. Add 20 kg of embedded bacteria into 80 kg of embedding agent solution and mix evenly to obtain a mixed solut...

Embodiment 2

[0042] An embedded and immobilized microbial fertilizer has the following raw material components: 10 kg of embedded bacteria and 90 kg of embedding agent.

[0043] Preparation method, concrete steps are as follows:

[0044] 1. Take the silicate bacteria solution in the logarithmic growth phase, centrifuge at 5000r / min for 20min in a centrifuge, weigh 10 kg of the precipitate, and obtain the embedded bacteria;

[0045] 2. Dissolve sodium alginate in water at a temperature of 70-90°C to make an aqueous solution with a concentration of 6%wt; take gelatin and dissolve it in water at a temperature of 70-90°C to make an aqueous solution with a concentration of 6%wt; Mix the above two aqueous solutions in a volume ratio of 1:1 to obtain an embedding agent solution;

[0046] 3. Take CaCl 2 Dissolve in water to make 2.2% CaCl by weight 2 aqueous solution;

[0047] 4. Add 10 kg of embedding bacteria into 90 kg of embedding agent solution and mix evenly to obtain a mixed solution fo...

Embodiment 3

[0052] The raw material components of an embedded and immobilized microbial fertilizer are as follows: 30 kg of embedded bacteria and 70 kg of embedding agent.

[0053] Preparation method, concrete steps are as follows:

[0054] 1. Take the Azotobacter rotundum bacteria solution in the logarithmic growth phase, centrifuge it on a centrifuge at 5000r / min for 20min, weigh 30kg of the precipitate, and obtain the embedded bacteria;

[0055] 2. Dissolve sodium alginate in water at a temperature of 70-90°C to make an aqueous solution with a concentration of 7%wt; take gelatin and dissolve it in water at a temperature of 70-90°C to make an aqueous solution with a concentration of 7%wt; Mix the above two aqueous solutions in a volume ratio of 1:1 to obtain an embedding agent solution;

[0056] 3. Take CaCl 2 Dissolve in water to make 2.2% CaCl by weight 2 aqueous solution;

[0057] 4. Add 30 kg of embedded bacteria into 70 kg of embedding agent solution and mix evenly to obtain a ...

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Abstract

The present invention relates to a microorganism fertilizer and the preparation thereof, especially to an embedded fixed microorganism fertilizer and the preparation method thereof, belonging to the field of the biological fertilizer technique. The microorganism fertilizer is composed of 10-30 weight parts embedded bacteria, 70-90 weight parts embedding agent; the fertilizer is prepared by instilling the mixed liquid of embedded bacteria and embedding agent into the CaCl2 water solution to prepare the embedded particle, and multiplication-propagating the embedded particle in the YMA culture solution. When the product of the invention is applied the crop production can be remarkably increased and the quality of the agricultural product can be improved, the soil component can be remarkably improved and the continuable utilizing capability of the soil can be increased.

Description

technical field [0001] The invention relates to a microbial fertilizer and its preparation, in particular to an embedded and immobilized microbial fertilizer and its preparation method, belonging to the technical field of biological fertilizers. Background technique [0002] Fertilizer is the "grain" of food. As an important member of the fertilizer family, microbial fertilizer plays an important role in agricultural production. Microbial fertilizer refers to a class of specific preparations containing living microorganisms, which are applied in agricultural production and can obtain specific fertilizer effects. The results of multi-point tests at home and abroad show that microbial fertilizers can increase production by 15% to 19% under different crops and agricultural conditions, and sorghum, pearl rice, etc. have obvious reactions, and 50% of the experiments inoculated with millet and sorghum have significantly increased production. From 1979 to 1986, Israel conducted in...

Claims

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Application Information

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IPC IPC(8): C05F11/08C12N11/04
Inventor 江丽华王梅刘兆辉林海涛王月明郑福丽宋效宗谭德水高新昊
Owner INST OF SOIL & FERTILIZER SHANDONG ACAD OF AGRI SCI
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