Broccoli male sterility line transfer and hybrid preparation method

Abstract

The invention discloses a transfer and hybrid compound method for the male sterile lines of broccoli, which is characterized in that, broccoli cytoplasmic male sterile heterozygote 725 is used as the female parent; broccoli inbred line 891 is used as the recurrent parent; after hybridization, all sterile plants with the pollen of flowers having no vigor are chosen to perform backcrossing; after 6 to 8 generations of transfer, broccoli cytoplasmic male sterile line green crown 1 is developed; all recurrent parents are the maintainer lines of corresponding cytoplasmic sterile lines 891; the hybrid compound method of the male sterile line of broccoli is that, cytoplasmic male sterile line green crown 1 is used as the female parent, and excellent early, middle and late inbred lines of broccoli are used as the male parent; the female parent and the male parent are respectively timely sown according to the ratio of 2 to 3:1; then first filial generation hybrid is prepared. The transfer and hybrid compound method for the male sterile line of broccoli has the advantages of low cost, high purity of the finished product and easy provenances.

Description

technical field [0001] The invention relates to a vegetable hybrid breeding method, in particular to a broccoli cytoplasmic male sterile line breeding and hybrid seed production method. Background technique [0002] Broccoli is a variety of Brassica oleracea in the family Cruciferae (Cruciferae), a variety with green or purple curds as the product, a one-year or two-year herbaceous plant. Scientific name: Brassica oleracea L. var. italica Planch. Chromosome number Zn=2x=18. Evolved from cabbage, the evolution center is the eastern Mediterranean coast. In the 1980s, my country began to introduce some foreign broccoli varieties; in the 1990s, the planting area of ​​broccoli grew rapidly, but domestic broccoli breeding lagged far behind other species of Brassicaceae. At present, the breeding in our country is mainly based on the traditional breeding methods of purification, rejuvenation and systematic breeding. The Institute of Vegetables and Flowers of the Chinese Academy ...

AI Technical Summary

Problems solved by technology

Broccoli has obvious heterosis. Self-incompatibility lines are mostly used for hybrid varieties, but self-incompatibility lines are labor-intensive and costly to breed parents, and the viability of self-trading for multiple generations has deteriorated. The rate is not stable enough and the purity cannot reach 100%

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