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Method for increasing lincomycin yield
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A technology of lincomycin and yield, which is applied in the biological field to achieve the effect of increasing yield
Inactive Publication Date: 2011-11-02
EAST CHINA UNIV OF SCI & TECH
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[0004] Foreign studies have found that directly adding propylproline as a precursor in the fermentation process can increase the yield of lincomycin A and correspondingly reduce the yield of lincomycin B, but whether adding other non-precursor amino acids will increase the yield of lincomycin The yield of mycin A has not yet been reported
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Embodiment 1
[0017] Embodiment 1, the impact of adding proline on the biosynthesis of lincomycin
[0018] 1.1. The effect of the amount of proline added on the biosynthesis of lincomycin
[0019] To prepare the fermentation medium for lincomycin, add 0.001%, 0.005%, 0.01%, 0.05%, 0.1%, and 0.2% proline to the medium by weight respectively, and at the same time culture without adding proline As a control, Streptomyces lincolnensis was inserted into Streptomyces lincolnensis for 168 hours of fermentation and culture, and then samples were taken to determine the titer of lincomycin. The results are shown in Table 1.
[0020] Table 1, the effect of the amount of proline added on the biosynthesis of lincomycin
[0021] Addition of proline (%)
[0022] It can be seen from the results in Table 1 that adding proline to the fermentation medium can obviously improve the biosynthesis of lincomycin, and the preferred adding amount is in the range of 0.005-0.01%.
[0023] 1.2. Effect of pro...
Embodiment 2
[0028] Embodiment 2, the impact of adding alanine on lincomycin biosynthesis
[0029] 2.1. The effect of the amount of alanine added on the biosynthesis of lincomycin
[0030] To prepare the fermentation medium of lincomycin, add alanine at a weight ratio of 0.005%, 0.01%, 0.03%, 0.05%, and 0.1% to the medium, and use the medium without adding alanine as a control , after inserting Streptomyces lincolnensis (Streptomyces lincolnensis) and fermenting for 168 hours, the potency of lincomycin was measured by sampling, and the results are shown in Table 3.
[0031] Table 3. The effect of the amount of alanine added on the biosynthesis of lincomycin
[0032] Addition amount of alanine (%)
0.005
0.01
0.03
0.05
0.1
control
Relative potency
106
111
99
72
19
100
[0033] It can be seen from the results in Table 3 that adding alanine to the fermentation medium can obviously improve the biosynt...
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Abstract
The invention provides a method of increasing lincomycin production, and the method is realized through adding proline and / or lactamine into a ferment culture medium of the lincomycin. By using the method provided by the method, the proline and / or the lactamine which are not used for precursor substance in the lincomycin synthesis are added into the ferment culture medium, yield of the lincomycin can be obviously increased.
Description
technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for increasing the yield of lincomycin. Background technique [0002] Lincomycin (LCM for short), also known as lincomycin, has a good effect on Gram-positive bacteria. It has no cross-resistance and has low toxicity, so it is widely used in Diseases caused by positive bacteria, at the same time, it also has a certain inhibitory effect on Gram-negative bacteria. Lincomycin and its downstream semi-synthetic products are called lincosamide antibiotics, and lincosamide antibiotics have become one of the main antibiotics in clinical application. [0003] Generally speaking, lincomycin refers to its A component, which is composed of coca leaf base PHA (trans-N-methyl-4-n-propyl proline (PPL)) and methionine. Amide MTL (6-amino-6,8-dideoxy-1-thio-O-erythro-2-D-galactopyranoside) is an antibiotic formed by connecting two parts through a peptide bond. Such as figure ...
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