Application of bdellovibrio to eliminating pathogen in freshwater product and culture water thereof

A technology for cultivating water bodies and Bdellovibrio, which is applied in biological water/sewage treatment, water/sludge/sewage treatment, bacteria, etc., can solve the problems of long development cycle and high cost of antibiotics, to eliminate residues and avoid food poisoning event, to ensure high-quality results

Inactive Publication Date: 2008-10-01
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The abuse of various antibiotics has led to the resistance of pathogenic bacteria to antibiotic

Method used

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  • Application of bdellovibrio to eliminating pathogen in freshwater product and culture water thereof
  • Application of bdellovibrio to eliminating pathogen in freshwater product and culture water thereof
  • Application of bdellovibrio to eliminating pathogen in freshwater product and culture water thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Application of three strains of Bdellovibrio randomly mixed in eliminating pathogenic bacteria in edible tilapia, giant river prawn, river clam and river crab

[0038] The application method includes the following specific steps and their conditions:

[0039] Step 1: Isolation and purification of Bdellovibrio

[0040] (1) Observation and identification of Bdellovibrio plaques

[0041] Pretreat the collected fresh water or sediment, inoculate Aeromonas hydrophila, proliferate Bdellovibrio, invert the double-layer plate, phage plaques appear on the double-layer plate containing the host bacteria, and pick the expanding single spot , and then according to the microbiological method of liquid proliferation culture and double-layer plate inspection, the single plaque that is constantly expanding is picked again, and repeated several times, until the single plaque is passed down to form a plaque with the same shape, size and transparency, that is, A pure strain of Bdellovib...

Embodiment 2

[0060] The application method of any three strains of Bdellovibrio swimming body mixture to eliminate Aeromonas hydrophila, Pseudomonas putida, Edwardsiella tarda and E. Including the following specific steps and their conditions:

[0061] Step one, two and step three (1) are all the same as embodiment 1

[0062] Step 3 (2) Elimination test of Bdellovibrio swimmers against Aeromonas hydrophila, Pseudomonas putida, Edwardsiella tarda and Escherichia coli during transportation of tilapia, Macrobrachium rosenbergii, river clams and river crabs

[0063] The test container, test water and test used tilapia, Macrobrachium rosenbergii, river clam, river crab specifications are all the same as in Example 1. Put 4 intact grids in each aquarium, put tilapia, giant giant rosenbergii, river clams and river crabs in each grid, and immediately put it flat in the aquarium after sealing the lid of the grid , Drain the excess water in the tank so that the water level just covers the grid. B...

Embodiment 3

[0068] The application of arbitrarily mixing three strains of Bdellovibrio swimmers to eliminate Aeromonas hydrophila, Pseudomonas putida, Edwardsiella tarda and E.

[0069] The application method includes the following specific steps and their conditions:

[0070] Step one, two and step three (1) are all the same as embodiment 1

[0071] Step 3 (2) Application of Bdellovibrio Swimming Body in Tilapia, Macrobrachium rosenbergii, River Clam and River Crab

[0072] Before the test, 12 breeding ponds (0.5m×0.5m×0.5m=125L) were temporarily built.

[0073] The culture ponds were divided into two series of test and control. The test water is unfiltered cultured fresh water, and 100L is injected into each pool. The specifications of tilapia, Macrobrachium rosenbergii, river clam and river crab used in the test are all the same as in Example 1. Put 20 tilapias, giant river prawns, river clams and river crabs in each pool. Before the experiment, it was sterilized by sodium dichlor...

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Abstract

The present invention provides an application of a bdellovibrio eliminating pathogenic bacteria in freshwater products and its aquaculture water, especially fit for eliminating pathogenic bacteria carried before freshwater products eating or processing, by transportation process or its aquaculture water. Bdellovibrio concentrated solution is added in aquaculture water, before eating, processing or in the course of transportation, the concentration achieves 10<3>pfu/ml. When the method is applied in freshwater products processing or in the course before processed, or in the course of transportation and in aquaculture water, the concentrations of bdellovibrio telotroch are 10<5>-10<13> pfu/ml, 10<4>-10<12> pfu/ml and 10 <3>-10<7> pfu/ml respectively. The clearance ratios of the bdellovibrio are all above 99%. The invention is fit for the whole process from freshwater products cultivation to the process before eating. The method not only improving the safety factor of consumer raw eating freshwater products, protecting health of consumer, but also playing an important role in green processing production of freshwater products.

Description

technical field [0001] The invention belongs to the field of biotechnology, relates to bacteria and its application, specifically refers to the application of Bdellovibrio in eliminating pathogenic bacteria in freshwater products and their cultured water bodies, and is especially suitable for eliminating freshwater products before consumption or processing, during transportation and their cultivation. Pathogenic bacteria carried in water bodies. Background technique [0002] Bdellovibrio (Bdellovibrio) is a kind of parasitic bacteria isolated from Pseudomonas beneficii ATCC11355 by Stolp and Petzhold to attack and lyse other bacteria to complete their own growth and reproduction. Bdellovibrio has a phage-like function, and has a good cracking and clearing effect on pathogenic bacteria that can cause fish farming diseases and human food poisoning, but has no toxic side effects on animals and plants. [0003] Aeromonas hydrophila, Pseudomonasputida, Edwardsiella tarda and Esc...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F103/22
Inventor 蔡俊鹏李娟
Owner SOUTH CHINA UNIV OF TECH
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