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Antioxidation active peptide and preparation thereof

A technology of antioxidant active peptides and neutral proteases, applied in the fields of peptides and fermentation, can solve the problems of hemoglobin antioxidant active peptides that have not yet been discovered, and achieve the effects of strong antioxidant capacity, reduced production costs, and low production costs

Inactive Publication Date: 2008-11-05
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there is no report on the preparation of hemoglobin antioxidant active peptides

Method used

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  • Antioxidation active peptide and preparation thereof
  • Antioxidation active peptide and preparation thereof
  • Antioxidation active peptide and preparation thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Take 5g of hemoglobin dry powder (prepared by centrifugal spray drying of pig blood), dissolve it in 100ml of distilled water, keep it warm at 90°C for 10min, then use 1N NaOH and 1N HCL to adjust the pH of the system to 7.0, then add 0.08g of AS1398 neutral protease ( (purchased from Beijing Donghua Qiangsheng Biotechnology Co., Ltd.), then hydrolyzed at 45°C and pH value of 7.0 for 4 hours, then kept at 90°C for 10 minutes, then desalted by ion exchange resin, concentrated, and freeze-dried to obtain antioxidant activity Peptide 3.1 g.

[0027] Determination of the content of antioxidant active peptides: Weigh the dry powder of the above antioxidant active peptides and dissolve them in distilled water. The peptide content is greater than 95% as measured by the biuret method, indicating that the prepared antioxidant peptides are of high purity.

[0028] Determination of the ash content of the antioxidant active peptide: Weigh the dry powder of the above antioxidant act...

Embodiment 2

[0030] Take 2g of dry hemoglobin powder (prepared by centrifugal spray-drying of pig blood), dissolve it in 40ml of distilled water, keep it warm at 90°C for 10min, then adjust the pH of the system to 7.0 with 1N NaOH and 1N HCL, then add 0.032g of AS1398 neutral protease (Beijing Donghua Qiangsheng Biotechnology Co., Ltd.), then hydrolyzed at 45°C and pH value of 7.0 for 3.5h, then kept at 90°C for 10min, then desalted by ion exchange resin, concentrated, and freeze-dried to obtain the antioxidant active peptide 1.3 g.

Embodiment 3

[0032] Take 8g of dry hemoglobin powder (prepared by centrifugal spray drying of pig blood), dissolve it in 160ml of distilled water, keep it warm at 90°C for 10min, then adjust the pH of the system to 7.0 with 1N NaOH and 1N HCL, then add 0.128g of AS1398 neutral protease (Beijing Donghua Qiangsheng Biotechnology Co., Ltd.), then hydrolyzed at 45°C and pH value of 7.0 for 4.5h, then kept at 90°C for 10min, then desalted by ion exchange resin, concentrated, and freeze-dried to obtain the antioxidant active peptide 4.8 g.

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Abstract

The invention discloses an anti-oxidative active peptide and a preparation method thereof, which mainly comprises the following steps of dissolving dry hemoglobin powder into water, then hydrolyzing with neutral protease, finally desalinating, concentrating, freezing and drying. The anti-oxidative active peptide of the invention has strong anti-oxidation capability. The purity of the invention is very high, which can reach more than 95 percent, with the ash content of less than 1.5 percent. The invention is extremely safe without side effect and undesirable odor. Besides anti-oxidation effect, the anti-oxidative active peptide of the invention also has the effects of immune regulation, lowering blood pressure and lowering cholesterol, etc. The low cost of the invention cuts down the production cost for more than 25 percent.

Description

technical field [0001] The invention belongs to the field of health care medicine or functional food bioengineering, and specifically relates to an antioxidant active peptide and a preparation method of the antioxidant active peptide Background technique [0002] Oxidation is a physiological mechanism required for cell survival, and this dependence of cells leads to the production of free radicals and reactive oxygen species. When the generation of free radicals exceeds a certain amount, their activity will be greater than that of cell protection enzymes, which will directly lead to the destructive lethal effect of cells (apoptosis), and the respiration of cells will also stop. Oxidation plays an important role in the aging of the human body and the occurrence of certain diseases. Free radicals produced by oxidation in the body can lead to atherosclerosis, diabetes and rheumatoid arthritis (Abuja, P et al. Clinica Chimica Acta, 2001, 306: 1-17. Halliwell, B. Cardiovascular ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K2/00C07K1/14C12P21/06
Inventor 罗永康孙骞沈慧星胡鑫
Owner CHINA AGRI UNIV
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