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Application of marsdenia tenacissima total saponin in preparing medicament for treating hepatitis B

A technology of total saponins and clearing vines is applied in the application field of preparing medicines for the treatment of hepatitis B, and can solve the problems of strong drug resistance, prone to rebound, high price and the like

Active Publication Date: 2010-12-08
北大世佳科技开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Interferon-α (IFN-α) was considered to be the only effective antiviral drug in the past, but it has limited indications, strong drug resistance, prone to rebound, high incidence of adverse reactions, and high price
In recent years, many nucleoside drugs have been found to have strong anti-HBV effects, such as lamivudine, acyclovir or its oral preparations famciclovir, Adefovir and Lobucavir, among which lamivudine has been studied more deeply. However, long-term application of lamivudine can cause mutations in the HBV DNA polymerase gene tyrosine-methionine-aspartic acid-aspartic acid (YMDD), making the virus resistant to lamivudine, making it difficult to popularize and apply
But there is no bibliographical report on the treatment of viral hepatitis B about the total saponins of Guanguan vine

Method used

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  • Application of marsdenia tenacissima total saponin in preparing medicament for treating hepatitis B
  • Application of marsdenia tenacissima total saponin in preparing medicament for treating hepatitis B
  • Application of marsdenia tenacissima total saponin in preparing medicament for treating hepatitis B

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Example 1 In vitro anti-hepatitis B virus (HBV) activity screening experiment of total saponins of Caulis officinalis

[0013] The contents of total saponins in the used total saponins of Caulis officinalis (CMT-S) are respectively 50%, 80%, and 95%.

[0014] The toxicity of CMT-S to 2.2.15 cells was detected by MTT method; the HBV-related antigen-surface antigen (HBsAg) in the cell culture supernatant after CMT-S with different saponin contents acted on 2.2.15 cells for different times was detected by ELISA and the level of e antigen (HBeAg), calculate the half toxic concentration of the drug to the cells (TC 50 ) and the half maximal inhibitory concentration (IC 50 ) to TC 50 and IC 50 The ratio (therapeutic index) to evaluate the effectiveness and safety of the drug. The experimental results are shown in Table 1.

[0015] Table 1. The non-toxic inhibition rate of CMT-S to hepatitis B virus HBsAg and HBeAg

[0016]

[0017] As can be seen from Table 1, after ...

Embodiment 2

[0018] Example 2 Experiment of the influence of total saponins of Tengguan vine on duck hepatitis B

[0019] The contents of total saponins in the used total saponins of Caulis officinalis (CMT-S) are respectively 50%, 80%, and 95%.

[0020] One-day-old shelducks were given tibial vein injections containing duck hepatitis B virus (DHBV) serum, and after replicating the shelduck viral hepatitis B model, CMT-S with different saponin contents were set up respectively (total saponin contents were 50%, 80% , 95%) three groups, according to the continuous administration of 80mg / kg for 10 days, respectively detect before administration (T 0 ), the 5th day after administration (T 5 ), Day 10 (T 10 ) and 3 days after drug withdrawal (P 3 ) DHBV-DNA level in duck serum, set up model control group (give physiological saline) and positive drug group (lamivudine 3TC, 20mg / kg) parallel operation simultaneously. Simultaneously calculate the drug T of each group of ducks 5 , T 10 and af...

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Abstract

The present invention relates to medicine application of marsdenia tenacissima total saponin. The marsdenia tenacissima total saponin can effectively cure virus b hepatitis, in particular can effectively restrain the reduplication of virus b hepatitis. The pharmaceutical preparation acceptable in clinic can be made from the marsdenia tenacissima total saponin and a variety of auxiliary materials acceptable in pharmacy for practical application.

Description

technical field [0001] The invention belongs to the field of research and development of new traditional Chinese medicines, and relates to the application of total saponins of vine vine in the preparation of medicines for treating hepatitis B. Background technique [0002] Hepatitis B is an inflammatory liver injury caused by hepatitis B virus (HBV), and is the most widespread and serious infectious disease in China. About 500,000 new cases of hepatitis B are reported in my country every year, accounting for about 1 / 4 of the total number of reported cases of Class A and B infectious diseases in the country. The prevalence of hepatitis B is a serious hazard to people's health, and the social and economic burden is heavy. Some hepatitis B virus carriers will develop liver disease and liver cirrhosis, and a small number of chronic liver disease patients will also develop hepatocellular carcinoma. According to the survey, there are currently about 20 million patients with chro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61P31/20A61K36/27A61K125/00A61P1/16
Inventor 周亚伟
Owner 北大世佳科技开发有限公司