Optimizing mass spectrogram model for detecting breast cancer characteristic protein and preparation method and application thereof

A technology of characteristic protein and mass spectrometry model, applied in the field of protein detection and mass spectrometry detection, can solve the problems of inability to detect low-abundance proteins, limited practical value, insufficient resolution, etc., to improve clinical cure, accurate design, reasonable and feasible, The effect of reducing the fatality rate
CN101329346AInactive Publication Date: 2008-12-24许洋
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
许洋
Publication Date
2008-12-24
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to an optimum mass spectrometry model and a preparation method thereof for detecting the feature protein of breast cancer, belonging to the field of mass spectrometry detection technique. The invention is characterized in that eight up-regulated proteins and three lower-regulated proteins are screened from the blood serum to be used as the feature proteins; any two or more proteins of the eleven proteins are chosen so as to establish a blood serum feature protein mass spectrometry model of identification with two in a group for patients with breast cancer and normal people, and patients with benign breast disease, lymphatic metastasis of breast cancer and remote metastasis of breast cancer according to the mass-charge ratio m / z of each protein peak and the critical peak average value of the protein; the preparation method of the invention provides a foundation for further discovering new breast cancer biological marks. The method of the invention is better than any single detection method adopted currently for the detection of the breast cancer, and provides a non-invasive technique for the early detection and early treatment of the breast cancer, thus providing a new method for reducing the mortality of the breast cancer, improving the cure rate of the breast cancer and screening and examining the breast cancer for high-risk population further.
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Description

technical field

[0001] The invention belongs to the technical field of mass spectrometry detection, in particular to a mass spectrometry detection method optimized for breast cancer blood. One captures biomarkers on a protein-binding magnetic bead matrix and detects breast cancer biomarkers using quantitatively controlled mass spectrometry. The invention mentioned here relates to the field of protein detection and is a new non-invasive in vitro mass spectrometry detection method. The present invention can be applied to the detection method or kit of the breast cancer biomarker combination in the body fluid that has been separated from the human body. Background technique

[0002] The occurrence of breast cancer is a process in which multiple gene mutations lead to inactivation of tumor suppressor genes and activation of oncogenes. Due to its own limitations, genomics research is difficult to fully elucidate the involvement of genes and proteins and their specific functions...

Claims

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