Method for detecting norethindrone and special ELISA kit thereof

An enzyme-linked immunosorbent reagent, norethisterone technology, applied in the field of detection of norethisterone, can solve the problems such as being unsuitable for on-site monitoring and screening of a large number of samples, high skill requirements for inspectors, and restrictions on popularization and application, so as to shorten the detection time. Time, ease of use, effect of simplified steps
CN101358965AInactive Publication Date: 2009-02-04贵州勤邦食品安全科学技术有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
贵州勤邦食品安全科学技术有限公司
Publication Date
2009-02-04
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention discloses a method of detecting norethisterone and a special enzyme linked immunosorbent kit thereof. The enzyme linked immunosorbent kit which is used for detecting the norethisterone comprises norethisterone haptens and specific antibodies of the norethisterone; and the specific antibodies are polyclonal antibodies or monoclonal antibodies of the norethisterone. The kit adopts the high specificity monoclonal antibodies of the norethisterone, which guarantees the reliability of detection results, and the experimental results show that the kit has the characteristics of high specificity, high sensitivity, high precision, high accuracy and the like; and the main reagents of the kit all adopt the form of working liquid, the operation is convenient, and the cost is low.
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Description

technical field

[0001] The invention relates to a method for detecting norethindrone and a special ELISA kit. Background technique

[0002] Norethindrone is a steroidal anabolic hormone with the structural formula figure 1 As shown, it is widely used to promote the growth of ruminants. However, studies have shown that norethindrone has obvious carcinogenicity, and people who eat livestock and poultry products containing this kind of drug are also prone to carcinogenicity. The use of norethindrone has been prohibited or strictly prohibited in Europe, America and other countries. The No. 235 document of the Ministry of Agriculture of my country stipulates that the residue limit of norethindrone in food of animal origin shall not be detected. Therefore, in practice, a method for detecting norethindrone with high precision and sensitivity is needed.

[0003] At present, the routine detection methods of norethindrone residues in animal tissues mainly include liquid chromatogr...

Claims

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