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Method for detecting norethindrone and special ELISA kit thereof

An enzyme-linked immunosorbent reagent, norethisterone technology, applied in the field of detection of norethisterone, can solve the problems such as being unsuitable for on-site monitoring and screening of a large number of samples, high skill requirements for inspectors, and restrictions on popularization and application, so as to shorten the detection time. Time, ease of use, effect of simplified steps

Inactive Publication Date: 2009-02-04
贵州勤邦食品安全科学技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, the routine detection methods of norethindrone residues in animal tissues mainly include liquid chromatography / mass spectrometry (LC / MS), high performance liquid chromatography (HPLC), etc., but these methods have complex equipment, cumbersome detection process and The shortcomings of high skill requirements for inspectors are not suitable for on-site monitoring and screening of a large number of samples, and the promotion and application are limited

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  • Method for detecting norethindrone and special ELISA kit thereof
  • Method for detecting norethindrone and special ELISA kit thereof
  • Method for detecting norethindrone and special ELISA kit thereof

Examples

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Embodiment 1

[0038] Example 1. Preparation and use of a kit using the conjugate of norethindrone hapten and carrier protein as the coating source and the enzyme-labeled secondary antibody as the enzyme marker

[0039] 1. The detection principle of the kit with norethindrone hapten and carrier protein conjugate as the coating source and the enzyme-labeled secondary antibody as the enzyme label is as follows:

[0040] When the coating on the microwell strip of the microtiter plate is originally the antigen of norethindrone hapten and carrier protein conjugate, add standard solution or sample solution to the microwell of the microtiter plate, and then add norethindrone specific Antibody, the residual norethindrone drug in the sample competes with the norethindrone-coupled antigen on the ELISA plate for the norethindrone-specific antibody, then the enzyme-labeled anti-antibody is added, and the color is developed with a chromogenic solution. The content of the drug is negatively correlated, an...

Embodiment 2

[0145] Embodiment 2, the kit for detecting norethindrone can also have following several kinds:

[0146] 1. The original coating is a specific antibody, and the enzyme marker is an enzyme-labeled norethindrone hapten kit

[0147] (1) The working principle of this kit is:

[0148] When the norethindrone-specific antibody is pre-coated on the microwell strip, after adding the sample solution or standard solution, add the enzyme-labeled norethindrone hapten solution. The norethindrone or norethindrone standard in the sample competes with the enzyme-labeled antigen for the norethindrone-specific antibody coated on the microtiter plate, and the color is developed with a chromogenic solution, and the absorbance value of the sample is related to the content of norethindrone in the sample Negatively correlated, compared with the standard curve, the content of norethindrone in the sample can be obtained. At the same time, the concentration range of norethindrone in the sample can be ...

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Abstract

The present invention discloses a method of detecting norethisterone and a special enzyme linked immunosorbent kit thereof. The enzyme linked immunosorbent kit which is used for detecting the norethisterone comprises norethisterone haptens and specific antibodies of the norethisterone; and the specific antibodies are polyclonal antibodies or monoclonal antibodies of the norethisterone. The kit adopts the high specificity monoclonal antibodies of the norethisterone, which guarantees the reliability of detection results, and the experimental results show that the kit has the characteristics of high specificity, high sensitivity, high precision, high accuracy and the like; and the main reagents of the kit all adopt the form of working liquid, the operation is convenient, and the cost is low.

Description

technical field [0001] The invention relates to a method for detecting norethindrone and a special ELISA kit. Background technique [0002] Norethindrone is a steroidal anabolic hormone with the structural formula figure 1 As shown, it is widely used to promote the growth of ruminants. However, studies have shown that norethindrone has obvious carcinogenicity, and people who eat livestock and poultry products containing this kind of drug are also prone to carcinogenicity. The use of norethindrone has been prohibited or strictly prohibited in Europe, America and other countries. The No. 235 document of the Ministry of Agriculture of my country stipulates that the residue limit of norethindrone in food of animal origin shall not be detected. Therefore, in practice, a method for detecting norethindrone with high precision and sensitivity is needed. [0003] At present, the routine detection methods of norethindrone residues in animal tissues mainly include liquid chromatogr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53G01N33/577G01N33/535C12N5/12C07J1/00
Inventor 沈建忠何方洋万宇平冯才伟赵正苗冯才茂汪善良罗晓琴陈炜玲
Owner 贵州勤邦食品安全科学技术有限公司
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