Detection kit for oral cavity mucous membrane tissue canceration trend detection and its use method
An oral mucosa and kit technology, applied in the field of molecular biology, can solve the problem of difficult diagnosis of lesions, and achieve the effect of short judgment time and high accuracy
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[0031] 1. Reagents and instructions included in the kit
[0032] The kit of the present invention contains 12 kinds of reagents, namely TRIZOL, chloroform, isopropanol, ethanol, Oligo(dT) 15 , Reagent I, Reagent II, Reagent III-a, Reagent III-b, Reagent III-c, Reagent III-d, and Reagent III-e. Among them, TRIZOL, chloroform, isopropanol, and ethanol are reagents for extracting total RNA from tissues, and Oligo(dT) 15 And reagent I is the cDNA reagent for reverse transcription of mRNA, reagent II is the PCR operation reagent of TaKaRa company, reagent III-a is the PCR primer of gene NF-1, reagent III-b is the PCR primer of gene ACP-2, reagent III- c is the PCR primer of the gene CTNN-B1, reagent III-d is the PCR primer of the gene SOCS-3, and reagent III-e is the PCR primer of the gene β-actin.
[0033] Details are shown in Table 1', wherein the specific components of Reagent I, Reagent II and Reagent III are shown in Tables 2'-4'.
[0034] Table 1': Reagents included in...
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