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Method for preparing fresh pilose antler active cell extract freeze-dried powder

A technology of extracting liquid and fresh velvet antler, which is applied in the field of preparation of fresh velvet antler active cell extract freeze-dried powder, which can solve the problems of decreased superoxide dismutase activity, large loss of velvet antler active components, and long extraction time , to achieve the effect of being conducive to modern industrial production, speeding up the extraction speed and preserving

Inactive Publication Date: 2009-04-22
郭凌云
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, due to the effect of high temperature, the active ingredients in deer antler lose a lot
In the processed velvet, the activities of superoxide dismutase (SOD) and alkaline phosphatase (AKP) decreased significantly.
In addition, according to the traditional extraction process, velvet slices are soaked in wine or extracted with organic solvents. Due to the microporous structure of velvet, it takes a long time for extraction, and at the same time, it will also cause pollution due to residual organic solvents.

Method used

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  • Method for preparing fresh pilose antler active cell extract freeze-dried powder

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Embodiment Construction

[0012] An example is given below to further illustrate the present invention: take 1 kilogram of fresh and frozen velvet antler and cut it into 2 mm thick antler slices, grind it for 20 minutes with a colloid mill, add 5 kilograms of ethanol with a concentration of 50%, stir evenly, and crush it with an ultrasonic crusher 20 minutes, and then centrifuged at 5000 rpm for 30 minutes to obtain supernatant and precipitate. Concentrate the supernatant part under reduced pressure, the temperature is less than 30 °C, concentrate to 1 / 5 of the original volume, after freezing, dry it into a dry powder through a freeze dryer; add 5 kg of ethanol with a concentration of 50% to the precipitate part, and heat to 60°C, ultrasonication for 20 minutes, and centrifugation at 5000rpm for 30 minutes to obtain supernatant and precipitate. Concentrate the supernatant under reduced pressure at a temperature lower than 30°C to 1 / 5 of the original volume. After freezing, dry it into a dry powder with...

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Abstract

The invention discloses a method for preparing freeze-dried powder of an active cell extract of fresh pilose antler. The preparation method comprises the following steps: fresh and frozen pilose antler is taken out, is sliced, is ground, is added with ethanol, is evenly stirred, is subjected to breaking treatment through an ultrasonic breaking instrument, and is centrifugated to obtain two parts of supernatant liquid and sediment; the part of the supernatant liqid is decompressed, condensed, frozen and dried through a freezedryer to prepare dry powder; and the sediment part is added with the ethanol, is heated and is subjected to ultrasonic breaking treatment and centrifugation. Through the three steps, a finished product can be obtained. The method uses the fresh pilose antler as a raw material, has stage extraction of low temperature and high temperature, ensures that enzyme and other biological active substances are not deactivated in the extraction process and simultaneously ensures the full extraction of other effective compositions. Through detection, the prepared product through the method has stable and high activity of SOD, which shows that the active substances in the cell are well stored and the effective compositions have high contents.

Description

technical field [0001] The invention relates to a preparation method of freeze-dried powder of cell extract, in particular to a preparation method of freeze-dried powder of active cell extract of fresh deer antler. Background technique [0002] Deer antler is a precious Chinese medicinal material, and at the same time, it is widely used as a nutritional tonic in the industries of health care products, food and cosmetics. The known nutrients in deer antler mainly include polypeptides, nucleotides, fatty acids, and some active enzymes and hormones. At present, the traditional method of processing velvet antler at home and abroad is to boil fresh velvet in boiling water after harvesting, then bake in an infrared oven at 70°C, and then air-dry. In this process, more blood loss. At the same time, due to the effect of high temperature, the active ingredients in velvet antler lose a lot. In the processed antler, the activities of superoxide dismutase (SOD) and alkaline phosphata...

Claims

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Application Information

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IPC IPC(8): A61K35/32
Inventor 郭凌云
Owner 郭凌云
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