Antibiotic tanshinone extract, preparation method thereof, use and products produced thereby

A kind of tanshinone extract, extract technology, applied in the field of medicine, can solve problems such as weak activity

Inactive Publication Date: 2009-04-22
BOTANIC CENTURY BEIJING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0034] According to previous studies and the results of the inventor's research on the anti-drug-resistant bacteria of the four main tanshinone components in Danshen, cryptotanshinone and dihydrotanshinone in the Danshen extract have the strongest antibacterial activity, while tanshinone IIA and tanshinone I less active

Method used

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  • Antibiotic tanshinone extract, preparation method thereof, use and products produced thereby
  • Antibiotic tanshinone extract, preparation method thereof, use and products produced thereby
  • Antibiotic tanshinone extract, preparation method thereof, use and products produced thereby

Examples

Experimental program
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Effect test

Embodiment 1

[0109] Example 1: 50 g of Salvia miltiorrhiza is crushed and extracted 3 times with 4 times the amount of 95% ethanol under reflux, each time for 1 hour. Combine the extracts. Concentrate and recover ethanol. The dregs were extracted twice with 4 times the amount of water, each time for 1 hour. The extract was dissolved in 15ml of water, and extracted 3 times with petroleum ether, 15ml each time. The aqueous portion was extracted three times with 15 mL of ethyl acetate. The ethyl acetate extracts were combined, concentrated, and dried to obtain 2.45 g of ethyl acetate extract. Among them, the content of total tanshinone is 18.38%, the content of cryptotanshinone is 3.74%, the content of tanshinone IIA is 8.56%, the content of tanshinone I is 4.64%, and the content of dihydrotanshinone is 1.44%.

Embodiment 2

[0110] Example 2: Put the pulverized Danshen medicinal material into an extraction kettle, set the pressure at 20 MPa and the temperature at 45°C. The flow rate of 95% ethanol solution was 1.0ml / min, mixed with CO2 and then circulated and dynamically extracted for 60min. The extract is separated to obtain a supercritical extraction extract. Among them, the content of total tanshinone is 48.45%, the content of cryptotanshinone is 5.26%, the content of tanshinone IIA is 39.40%, the content of tanshinone I is 2.75%, and the content of dihydrotanshinone is 1.04%.

Embodiment 3

[0111] Example 3: Take 1000 g of Danshen medicinal material, soak it with an appropriate amount of 95% ethanol for 24 hours, put it evenly into a percolation cylinder, and extract by percolation. The amount of percolation liquid collected is 12 times that of the medicinal material, and the percolation rate is 900 ml per hour. The percolation extract was concentrated under reduced pressure to recover ethanol to obtain 56 g of Danshen extract. Among them, the total tanshinone content is 14.0%, the cryptotanshinone content is 3.56%, the tanshinone IIA content is 5.92%, the tanshinone I content is 2.79%, and the dihydrotanshinone content is 1.74%.

[0112] Among the above three examples, Example 1 adopted the method of ethanol reflux extraction, Example 2 adopted the method of carbon dioxide supercritical extraction, although Example 3 carried out percolation extraction, but no recrystallization, the obtained extract content Neither meet the requirements. In the following example...

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Abstract

The invention relates to an antibacterial tanshinone extract extracted from Salvia spp. roots, a preparation method thereof, application of the antibacterial tanshinone extract and products of the antibacterial tanshinone extract. The extract contains cryptotanshinone, dihydro-tanshinone, tanshinone I and tanshinone IIA, wherein the total weight content of the four compounds is 40 to 99 percent of the total weight content of the extract; the weight content of the cryptotanshinone is 50 to 95 percent of the total weight of the four compounds; the weight content of the dihydro-tanshinone is 5 to 30 percent of the total weight of the four compounds; the weight content of the tanshinone I is 0 to 20 percent of the total weight of the four compounds; and the weight content of the tanshinone IIA is 0 to 40 percent of the total weight of the four compounds. The preparation method comprises the steps of diacolation extraction and purification for two times. The extract is used for preparing antibacterial medicines or other products. The tanshinone extract obtained has high purity, more reasonable proportions of various compositions, and can exert better clinic effect.

Description

technical field [0001] The invention relates to the field of medicine, in particular to an antibacterial tanshinone extract, its preparation method, application and its product. Background technique [0002] Salvia miltiorrhiza (Latin scientific name: Radix Salviae Miltiorrhizae, English name: Salviaroot) is one of the earliest and most widely used herbal medicines in traditional medicine in my country, and is listed as top grade in "Shen Nong's Herbal Classic". The medicine has the effects of dispelling blood stasis and relieving pain, promoting blood circulation and promoting menstrual flow, clearing away heart-fire and relieving restlessness. It is mainly used to treat irregular menstruation, accumulation of lumps in the abdomen, heat arthralgia pain, sore swelling and pain, upset and insomnia, hepatosplenomegaly, etc. The source of medicinal materials included in "Chinese Pharmacopoeia" is the dry root and rhizome of Salvia miltiorrhiza Bge. However, some plants of the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/53A61K31/58A61K8/63A61P31/04A61P17/10A61Q19/00
CPCA61K36/537A61K31/343A61P17/10A61P31/04
Inventor 钟忠
Owner BOTANIC CENTURY BEIJING
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