Salt resistant endo cellulose and coding gene thereof

An endocellulase and coding gene technology, applied in the field of genetic engineering, can solve the problems of low activity, difficult industrial application, poor stability, etc.

Inactive Publication Date: 2010-12-08
HUAZHONG AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the cellulase genes that have been cloned are difficult to be applied industrially due to their low activity or poor stability.

Method used

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  • Salt resistant endo cellulose and coding gene thereof
  • Salt resistant endo cellulose and coding gene thereof
  • Salt resistant endo cellulose and coding gene thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 1. Construction of Halomonas sp.S66-4 DNA library and screening of endocellulase-producing positive clones

[0020] 1. Extract bacterial chromosomal DNA:

[0021] (1) The bacterial strain used for cloning the gene comes from a strain of marine microorganism HHalomonas sp provided by the Third Oceanographic Research Institute of the State Oceanic Administration of Xiamen City, Fujian Province, China, and the bacterial strain number is S66-4 (the preservation number of the institute is MCCC 1AOS66- 4. The bacterial strain is a general-purpose bacterial strain that can be distributed by the Institute), and high-salt LB liquid medium (medium composition: 1% peptone, 0.5% yeast culture and 2% sodium chloride, supplemented with water to 1L , adjust the pH to 7.0) after culturing at 28°C for 24 hours, take 1.5ml of the bacterial solution in a sterilized Ep tube, centrifuge at 12,000 rpm for 1 minute, discard the supernatant, and collect the bacterial cells;

[0022] (2) Wash ...

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Abstract

The invention belongs to the technical field of gene engineering, in particular to a new salt resistant endo cellulase and a coding gene thereof. The invention discloses a sequence and a coding area of the gene; the sequence of the gene is shown as SEQ ID NO: 1; the full length of the sequence is 1053bp; and 350 amino acids are coded. Escherichia coli DH5 Alpha / S664 / 6p / cel8h containing a gene plasmid is preserved in China Center for Type Culture Collection (CCTCC); and the serial number of preservation is CCTCC NO:M208233. Biology validates and shows that the endo cellulase has strong tolerance to high saline salinity. The endo cellulase can be widely applied in high saline catalysis, cellulose fermentation and other industrial fields.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to a new salt-tolerant endo-cellulase and the cloning of its coding gene. technical background [0002] In today's world, energy problems are becoming more and more serious. Searching for sustainable bio-energy has become the focus of competition among countries. Cellulose accounts for the vast majority of the Earth's total biomass and is one of the most promising bioenergy sources existing on Earth (Lynd et al. 2005). Cellulose is a linear polysaccharide chain formed by the oligomerization of glucose units. In organisms, it is mostly cross-linked with hemicellulose and lignin to form a network lattice structure. Degrading cellulose to form glucose and oligosaccharides that can be fermented industrially is the core of cellulose utilization. [0003] The enzymatic degradation of cellulose is mainly completed by the synergistic action of exo-cellulase, endo-cellulase, a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12R1/19C12N9/42C12N1/21C12N15/56
Inventor 刘子铎黄小罗洪玉枝邵宗泽
Owner HUAZHONG AGRI UNIV
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