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Method for rapid measurement of cistanche salsa seed vigor

A technology for rapid determination of cistanche, applied to germination equipment, etc., can solve the problems of long measurement time, unreliable results, complicated operation, etc., and achieve the effect of simple method, fast measurement speed, accurate and reliable results

Inactive Publication Date: 2009-05-13
INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the disadvantages of long measurement time, complicated operation and unreliable results in the existing Cistanche seed viability measurement, and provide a method for quickly measuring Cistanche seed viability

Method used

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  • Method for rapid measurement of cistanche salsa seed vigor
  • Method for rapid measurement of cistanche salsa seed vigor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Determination of Viability of Cistanche Seeds

[0023] Select Cistanche seeds with a diameter greater than 0.5mm (collected in 2006 at the planting base of Herbal Cistanche in Yongning, Ningxia), and divide them into 2 replicates, each with 50 seeds, place them on white paper and gently rub the outer seeds with filter paper. The skin was rubbed and removed, and the seeds of Cistanche deserticola were transferred to a small tube on a 96-well culture plate, and then added with a concentration of 0.3% tetrazolium staining solution (0.7 gram of tetrazolium powder (purchased from Beijing Mashi Fine Chemicals Co., Ltd.) Mix and dissolve 100ml of the prepared phosphate buffer solution, put it into a brown glass bottle, store it at 4°C for later use), then place it in an incubator at 38°C for 15 hours; pour off the tetrazolium staining solution, and wash Rinse twice, and then observe the seeds of Cistanche deserticola under a dissecting microscope. As a result, the nu...

Embodiment 2

[0024] Example 2 Determination of viability of Cistanche cistanche seeds

[0025] Select Cistanche tuberosa seeds with a diameter greater than 0.4mm (collected in Hotan, Xinjiang in 2004), and set up 2 replicates, each with 50 seeds, place them on white paper and gently rub the outer testa with filter paper. Knead and remove, transfer the obtained Cistanche cistanche seeds to the small tube on the 96-well culture plate, add 0.5% tetrazolium staining solution (dissolve 0.5 g tetrazolium powder in the phosphate buffer of 100 ml, fully dissolve, stir well ), then soaked and dyed at 43°C for 8 hours, poured off the dyeing solution, washed the seed kernels twice with phosphate buffer solution, and then observed the seeds of Cistanche tubulosa under a dissecting microscope, and the result was that the mesoderm of two replicates was dyed red The numbers were 23 and 21 respectively, and the average proportion of viable seeds was calculated to be 44%.

Embodiment 3

[0026] Example 3 Cistanche seed viability test

[0027] Select Cistanche seeds with a diameter greater than 0.5 mm (collected in Yongning, Ningxia, Herbal Cistanche planting base in 2005), set up 2 replicates, 50 seeds for each replicate, place the Cistanche seeds on white paper and gently rub the outer testa with filter paper. Grind and remove, transfer the clean seed kernels to a small tube on a 96-well culture plate, add 0.7% tetrazolium staining solution (dissolve 0.3 g of tetrazolium powder in 100 ml of prepared phosphate buffer, fully dissolve, stir well ), then stained at 47°C for 6 hours, poured out the TTC staining solution, washed twice with buffer solution or distilled water, and then observed Cistanche seeds under a dissecting microscope. Yutian Image Technology Co., Ltd.) counts on the computer after taking pictures, and calculates the result. Specific operation: Install the Anymicro DSS microscopic digital photography system on the Lycra mirror (S8APO), install ...

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Abstract

The invention discloses a quick measurement method of broomrape seed vitality, belongs to the medical plants domain, comprising the following steps: peeling the broomrape seed by kneading; soaking and dyeing the broomrape kernel in TTC solution with weight percentage of 0.3-1.0% at 38-48 degree for 6-15h; dumping the TTC solution and flushing the broomrape kernel for 2-3 times using the flushing liquor; observing the broomrape kernel under the anatomical lens; counting the kernel with red germ, namely seed with vitality. The quick measurement method is accurate and reliable and solves the prediction problem of broomrape seed germination ratio to save the seeds; and then the measurement method has quick measurement speed and is suitable for large-scale detection; in addition the method is simple, suitable for generalizing and applying.

Description

technical field [0001] The invention belongs to the field of medicinal plants, and in particular relates to a method for quickly measuring the viability of Cistanche deserticola seeds. Background technique [0002] Cistanche deserticola is a traditional Chinese herbal medicine, which has the functions of tonifying kidney, benefiting essence, strengthening tendons and strengthening marrow. In recent years, it is often used as an anti-aging and anti-cancer drug. With the continuous improvement of living standards, people's awareness of self-care has increased, and health care products based on Cistanche are popular among people, and the demand for Cistanche is also increasing, resulting in the predatory mining of wild Cistanche resources. , which has not only destroyed non-renewable wild resources, but also destroyed the ecological environment, but it is still difficult to meet people's needs. Artificial cultivation of Cistanche deserticola is the main way to solve this probl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/02
Inventor 陈君徐荣刘同宁
Owner INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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