Method for rapid measurement of cistanche salsa seed vigor
A technology for rapid determination of cistanche, applied to germination equipment, etc., can solve the problems of long measurement time, unreliable results, complicated operation, etc., and achieve the effect of simple method, fast measurement speed, accurate and reliable results
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0022] Example 1 Determination of Viability of Cistanche Seeds
[0023] Select Cistanche seeds with a diameter greater than 0.5mm (collected in 2006 at the planting base of Herbal Cistanche in Yongning, Ningxia), and divide them into 2 replicates, each with 50 seeds, place them on white paper and gently rub the outer seeds with filter paper. The skin was rubbed and removed, and the seeds of Cistanche deserticola were transferred to a small tube on a 96-well culture plate, and then added with a concentration of 0.3% tetrazolium staining solution (0.7 gram of tetrazolium powder (purchased from Beijing Mashi Fine Chemicals Co., Ltd.) Mix and dissolve 100ml of the prepared phosphate buffer solution, put it into a brown glass bottle, store it at 4°C for later use), then place it in an incubator at 38°C for 15 hours; pour off the tetrazolium staining solution, and wash Rinse twice, and then observe the seeds of Cistanche deserticola under a dissecting microscope. As a result, the nu...
Embodiment 2
[0024] Example 2 Determination of viability of Cistanche cistanche seeds
[0025] Select Cistanche tuberosa seeds with a diameter greater than 0.4mm (collected in Hotan, Xinjiang in 2004), and set up 2 replicates, each with 50 seeds, place them on white paper and gently rub the outer testa with filter paper. Knead and remove, transfer the obtained Cistanche cistanche seeds to the small tube on the 96-well culture plate, add 0.5% tetrazolium staining solution (dissolve 0.5 g tetrazolium powder in the phosphate buffer of 100 ml, fully dissolve, stir well ), then soaked and dyed at 43°C for 8 hours, poured off the dyeing solution, washed the seed kernels twice with phosphate buffer solution, and then observed the seeds of Cistanche tubulosa under a dissecting microscope, and the result was that the mesoderm of two replicates was dyed red The numbers were 23 and 21 respectively, and the average proportion of viable seeds was calculated to be 44%.
Embodiment 3
[0026] Example 3 Cistanche seed viability test
[0027] Select Cistanche seeds with a diameter greater than 0.5 mm (collected in Yongning, Ningxia, Herbal Cistanche planting base in 2005), set up 2 replicates, 50 seeds for each replicate, place the Cistanche seeds on white paper and gently rub the outer testa with filter paper. Grind and remove, transfer the clean seed kernels to a small tube on a 96-well culture plate, add 0.7% tetrazolium staining solution (dissolve 0.3 g of tetrazolium powder in 100 ml of prepared phosphate buffer, fully dissolve, stir well ), then stained at 47°C for 6 hours, poured out the TTC staining solution, washed twice with buffer solution or distilled water, and then observed Cistanche seeds under a dissecting microscope. Yutian Image Technology Co., Ltd.) counts on the computer after taking pictures, and calculates the result. Specific operation: Install the Anymicro DSS microscopic digital photography system on the Lycra mirror (S8APO), install ...
PUM
Login to view more Abstract
Description
Claims
Application Information
Login to view more - R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap