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Novel sensitive enhancement and synergistic substance for chemical treatment of tumor

An anti-tumor drug and tumor technology, applied in the field of pharmacology, can solve the problems of increasing the burden of chemotherapy patients and increasing the number of chemotherapy drugs

Inactive Publication Date: 2009-05-20
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The use of some preparations that have a reversal effect on MDR, although it can improve the anticancer activity of chemotherapy drugs, it also increases the number of chemotherapy drugs and increases the burden on chemotherapy patients

Method used

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  • Novel sensitive enhancement and synergistic substance for chemical treatment of tumor
  • Novel sensitive enhancement and synergistic substance for chemical treatment of tumor
  • Novel sensitive enhancement and synergistic substance for chemical treatment of tumor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Preparation of geraniol

[0065] Geraniin brevifolia was purchased from the Key Laboratory of Traditional Chinese Medicine Resources and Chemistry of Hubei Province; in addition, it can also be obtained according to Taylor, R.S., etc. (1998), Anti-bacterial constituents of the Nepalese medicinal herb, Centipeda minima.Phytochemistry.47, 631-634 Prepared by the method provided in .

Embodiment 2

[0067] Cytotoxic effect of geraniol on MDR cells and drug-sensitive cells

[0068] In this example, the cytotoxic effects of geraniol on MDR cells and drug-sensitive cells were compared. Cytotoxic effect of MDR cell HCT-8 / VCR (purchased from Xiangya Central Laboratory of Central South University, HTCC-90) and drug sensitive cell HCT-8 (purchased from Xiangya Central Laboratory of Central South University, HTCC-86). HCT-8 / VCR and HCT-8 are two representative tumor cell models randomly selected by the inventors.

[0069] The geranilin was diluted with RPMI1640 culture medium to an appropriate concentration. The cytotoxic effect of geranidin MDR cells (HCT-8 / VCR) and drug-sensitive cells (HCT-8) was determined by rapid colorimetric method of thiazolium blue (MTT).

[0070] Cells in logarithmic growth phase 10 4Cells / ml were inoculated in a 96-well culture plate, 0.2ml per well, and a certain concentration of geraniin was added to make the final concentration of geraniin 4μM, 8...

Embodiment 3

[0074] Comparison of resistance multiples between geraniol and traditional tumor chemotherapeutic drugs

[0075] Adopt MTT method (specific experiment operation with reference to embodiment 2) to investigate five kinds of clinically more commonly used anticancer drugs vincristine (VCR), cisplatin (DDP), 5-fluorouracil (5FU), mitomycin (MMC) and The cytotoxicity of hydroxycamptothecin (HEPT) to HCT-8 and HCT-8 / VCR under different concentrations and a certain action time (24h) was plotted with different concentrations of the drug and the survival rate of the cells to determine the half inhibitory concentration of the cells ( IC 50 ) to calculate the resistance factor (Resistance Factor, RF=IC50(HCT-8 / VCR) / IC50(HCT-8)). In addition, the drug resistance multiples of the five traditional tumor chemotherapy drugs were compared with Brevilin at the same action time.

[0076] Table 1 shows the comparison results of geraniin brevifolia and traditional tumor chemotherapeutic drugs.

...

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Abstract

The invention belongs to the field of pharmacology, and discloses new application of brevifolin used for preparing a sensitizer or a reversal agent of an antitumor drug. The invention discloses the new application of the brevifolin in improving the sensibility of tumor to the antitumor drug, and can effectively reverse multi-drug resistance of the tumor to the anti-tumor drug. Furthermore, the brevifolin has low toxicity and abundant resource, and has excellent medicinal prospect.

Description

technical field [0001] The invention belongs to the field of pharmacy, and more specifically, the invention relates to a novel sensitizing and synergizing substance useful for tumor chemotherapy and its application. Background technique [0002] Multidrug resistance (MDR) refers to the cross-resistance to a variety of natural antitumor drugs with different structures and mechanisms of action when cancer cells are exposed to a natural antitumor drug and develop drug resistance. Medicinal properties. Cancer patients often develop multidrug resistance after chemotherapy. Multidrug resistance is the main reason for the failure of cancer chemotherapy, and it is also one of the problems that need to be solved urgently in cancer chemotherapy. The formation mechanism of multidrug resistance is complex. Tumor cells can lead to the production of MDR through different ways, and at the same time, a single MDR cell can have multiple drug resistance mechanisms at the same time. Any co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/365A61K45/06A61K31/407A61K31/475A61K31/513A61K31/704A61K33/24A61K9/14A61K9/20A61K35/00
Inventor 刘建文李长龙刘焱文吴和珍杨艳芳吴宏忠孙丽娟邓皖利徐文房一时山冬梅于有军王芳王劲
Owner EAST CHINA UNIV OF SCI & TECH
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