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Novel mutation involved in increased tolerance to imidazolinone herbicides in plants

A technology of imidazolinones, herbicides, applied in the field of novel mutations involving increased tolerance to imidazolinones herbicides in plants, capable of solving undescribed problems

Inactive Publication Date: 2009-05-27
BASF AGROCHEMICAL PROD BV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The prior art also does not describe imidazolinone-tolerant wheat or triticale plants comprising at least one altered Als nucleic acid from common wheat (Triticum aestivum) Shiloh cultivar

Method used

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  • Novel mutation involved in increased tolerance to imidazolinone herbicides in plants
  • Novel mutation involved in increased tolerance to imidazolinone herbicides in plants
  • Novel mutation involved in increased tolerance to imidazolinone herbicides in plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0179] Mutagenesis and Selection of Tolerant Wheat Lines

[0180] A sample of 1,500 Shiloh seeds was placed in a 1,000 ml beaker and covered with deionized water to at least 1 inch above the seed level. The beaker was then placed in the refrigerator at 4°C for 15-20 hours. The seed samples were taken out of the refrigerator and warmed to room temperature over a period of approximately 3 hours by placing the beaker at room temperature. In some cases, the heating process is accelerated by adding deionized water to the beaker.

[0181] Pour the deionized water from the seeds and pour the sodium azide solution into the beaker to at least 1 inch above the level of the seeds. Prepare the sodium azide solution as follows: add 27.218g KH to 1,500ml deionized water 2 PO 4 , Use thick H 3 PO 4 The solution was brought to pH 3 and the final solution was brought to a volume of 2 L with deionized water. Just before use, add 0.2604g NaN 3 , And keep the solution in the dark. After adding the so...

Embodiment 2

[0193] Molecular characterization of Shiloh-8 wheat strain

[0194] Subsequent molecular characterization revealed that the Shiloh-08 strain had a new adenine to guanine base pair substitution at position 142 of the Als1 gene. The mutant Als1 polynucleotide encodes a mutant AHAS polypeptide, which has an alanine to threonine substitution at position 96 corresponding to the wild-type AHAS polypeptide, and confers resistance to imidazolinone herbicides to the plant Resistance. The Shiloh-8 strain does not have mutations in Als2 or Als3 genes.

Embodiment 3

[0196] Characterization of imidazolinone tolerance traits of Shiloh-8 strain

[0197] Then, the tolerance of agronomic and related imidazolinone herbicides (imazamox) was evaluated under field conditions. Table 4 summarizes the yield and agronomic evaluation, which compares Shiloh-8 and wild-type Shiloh plants. These trials are typical cereal evaluation experiments conducted with an incomplete randomized block triple design. At harvest, the plot is 1.54m×4.62m. Table 5 summarizes the tolerance comparison between Shiloh-8 and the standard tolerance control 9804 for multiple imidazolinone herbicides (imazamox). These plots vary from a single row of 1m to larger plots of 1.54m×4.62m.

[0198] Table 4

[0199] Plant 5 (1bs / bu) Facultative Heading 4 / 12 / 01 Height Soil Wheat Husk Needle Bacteria Brown Spot Striped Rust

Loc test weight of ZADO virus spore stripe disease

Yield (1-9) KS (Septoria

tritici) Shiloh 67.6 58.9 6 108 52 36.3 1 5 6 4 3

Shiloh-8 61.5 59.1 6 109...

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Abstract

The present invention is directed to nucleic acids encoding polypeptides that confer upon a plant tolerance to an imidazolinone and / or other acetohydroxyacid synthase (AHAS) inhibiting herbicide when expressed in the plant. The present invention also provides plants having increased tolerance to an imidazolinone and / or other AHAS-inhibiting herbicide. More particularly, the present invention includes plants containing at least one IMI nucleic acid. The present invention also includes seeds produced by these plants and methods of controlling weeds in the vicinity of these wheat plants.

Description

Invention field [0001] The present invention generally relates to plants with increased tolerance to imidazolinone herbicides. More specifically, the present invention relates to plants obtained by mutagenesis, cross breeding and transformation, which have increased tolerance to imidazolinone herbicides. Background of the invention [0002] The acetohydroxy acid synthase (AHAS; EC 4.1.3.18; acetolactate synthase (ALS)) encoded by the Als nucleic acid is the first to catalyze the biochemical synthesis of branched-chain amino acids such as valine, leucine and isoleucine. An enzyme (Singh BK, 1999, Biosynthesis of valine, leucine and isoleucine in: Singh, BK(Ed) Plant aminoacids. Marcel Dekker Inc. New York, New York, pages 227-247). AHAS is the site of action of four structurally different herbicide families, including sulfonylureas (LaRossa RA and Falco SC, 1984, Trends Biotechnol. 2:158-161), imidazolinone (Shaner et al., 1984, Plant Physiol. 76:545-546), triazolopyrimidines (Sub...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/60C12N15/82C12N9/88C12N5/10C12N1/15C12N1/19C12N1/21A01H1/00A01H1/02A01H5/00A01H5/10
CPCC12N15/821C12N9/1022C12N15/8274
Inventor J·莫法特R·布伦斯I·伯克B·辛格
Owner BASF AGROCHEMICAL PROD BV
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