Osthole and carbendazim compound composition and use thereof
A compound composition, osthole technology, applied in the application, fungicide, biocide and other directions, can solve the problems of long cycle, high development cost of new pesticides, unrealistic apples, etc., achieve high safety, reduce resistance, etc. Drug-related risks and effects on reducing selection pressure
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Embodiment 1
[0018] Indoor toxicity determination and compounding ratio screening
[0019] 1.1 Test agents
[0020] 94.7% Osthole, provided by the Institute of Food Quality and Safety, Jiangsu Academy of Agricultural Sciences. Dissolve in 95% alcohol and prepare 10000mg / L mother liquor for later use.
[0021] 98% carbendazim, provided by Shandong Huayang Pesticide Chemical Group Co., Ltd. Use 0.1mol / L hydrochloric acid to make 10000mg / L mother liquor for later use.
[0022] The osthol products with a content of 10 to 90% from my country Yuancheng Science and Technology Development Co., Ltd., Shaanxi Hebo Natural Products Co., Ltd., Xi’an Sanwei Biotechnology Co., Ltd. and other manufacturers meet the requirements of the osthol used in the compound composition of the present invention. Requirements; and the carbendazim compounded with osthole is a commercially available pesticide product, as long as it meets the compounding ratio and application conditions of the present invention.
[0023] 1.2...
Embodiment 2
[0060] Field trial
[0061] According to the indoor compounding ratio screening experiment, osthol: carbendazim=1:35 is the best ratio. When osthol is 1mg, carbendazim is 35mg. We have made a 36% Osthole·Carbendazim suspension.
[0062] 2.1 Test reagents and community arrangement
[0063] According to "Part 118 of "Pesticide Field Efficacy Test Guidelines (II)" edited by the Ministry of Agriculture Pesticide Inspection Institute: Fungicides to prevent and cure apple rot disease" 36% Osthole·Carbendazim suspension is designed to prevent apple rot disease Field efficacy test.
[0064] 2.1.1 Test agents
[0065] 1% Osthole water emulsion 200 times. (Provided by Jiangsu Academy of Agricultural Sciences)
[0066] 50% carbendazim suspension 500 times. (Provided by Jiangsu Taicang Pesticide Factory)
[0067] 36% Osthole·Carbendazim Suspension Concentrate 400, 500, 600 times. (Prepared by Yantai Academy of Agricultural Sciences)
[0068] 2.1.2 Control agent
[0069] 10% Shigao water dispe...
Embodiment 3
[0083] Safety test
[0084] The content of carbendazim in the compound composition is 15% less than that of the commercially available products, so its toxicity to humans and animals and safety to crops are in line with the requirements; the content of osthole in the compound composition is 1%. The toxicity test results of accredited institutions show that 1% Osthole Water Emulsion is a slightly toxic pesticide:
[0085] Acute oral toxicity LD50 in rats >5000mg / kg, ♀>5000mg / kg;
[0086] Acute dermal toxicity in rats LD50 >5000mg / kg, ♀>5000mg / kg;
[0087] Rabbit eye irritation score is 0 (1h), after 48h, it is 0 (1:100) dilution, no irritation to eyes;
[0088] The rabbit eye irritation score is 0 (4h), which is non-irritating to the skin.
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