Freshwater reeds non-callus culture method

Abstract

The invention belongs to the technical field of plant tissue culture. A reed stem is used to establish a rapid in-vitro propagation method through the non-callus culture and obtain a complete high quality reed plant. The method has a simple process, a short propagation cycle, the inductivity as high as 92.3 percent, and the transplantation survival rate as high as 90.2 percent. The method can be used to supply high quality reed germchits rapidly, meet the increasingly improved market demand, and give full play to the actions of the reed in the ecological environment construction, water environment comprehensive treatment and wetland restoration projects.

Description

1. Technical field [0001] The invention belongs to the technical field of plant tissue culture. 2. Background technology [0002] The natural population of reed (Phragmites australis) mostly occurs in shallow wetlands, often in ponds, along rivers, tidal flats, and watery areas near rivers and streams. Reed is an economic crop, and its stalks can be used to make paper and rayon, and weave reed mats and straw mats; reeds in the growing period contain a lot of protein and sugar, and are excellent feed; reed leaves, flowers, stems, roots, and bamboo shoots can be used as medicine. At the same time, reed has important ecological functions, such as protecting soil and embankment, reducing waves and slowing flow, promoting siltation and preventing corrosion, etc.; In the restoration and reconstruction of river banks and marsh wetlands, reeds have become the must-selected plants and are planted on a large scale. With the implementation of a large number of ecological environment ...

AI Technical Summary

Problems solved by technology

With the implementation of a large number of ecological environment construction, water environment comprehensive management and wetland restoration projects in our country, the demand for reed seedlings and provenance will become more and more urgent, and the current technology will be difficult to meet the increasing market demand; therefore, the invention of a A method that can quickly provide high-quality reed seedlings is an inevitable need

[0003] After searching the China Patent Network and related paper websites, it is found that there are some reports on reed tissue culture, such as Wu Guoliang et al. in "Formation of Reed Embryogenic Callus and Plant Regeneration" using seeds to obtain complete plants through callus culture , but the callus cycle is longer and the process is more complicated; Wang Xiyou only studied the growth of callus in "Research on the growth of reed embryogenic callus", and did not grow a complete plant; Guo Rui wrote in "Salt-tolerant reed In "Studies on Somatic Embryogenesis and Plant Regeneration of Stem Tissues", plants are also obtained through callus culture, which also has the problem of a long period; in addition, in the patent "Asexual Propagation Method of Salt-tolerant Phragmites" by Yang Yingen, etc., It also describes the process of obtaining complete plants through callus culture, which also has the problem of a long period