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Colloidal gold chromatography test paper strip for quickly detecting perfluoro caprylic acid and preparation method thereof

A technology of perfluorooctanoic acid and detection test paper, which is applied in the detection field of biological immune methods, can solve the problems of application limitation of ELISA method, complicated operation process, etc., and achieves the effects of easy promotion, broad market prospect and cost saving.

Inactive Publication Date: 2009-09-16
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the ELISA method requires a matching microplate reader and supporting reagents, the operation process is still relatively complicated, and it is currently in the research and development stage at home and abroad, so the application of the ELISA method is greatly restricted

Method used

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  • Colloidal gold chromatography test paper strip for quickly detecting perfluoro caprylic acid and preparation method thereof
  • Colloidal gold chromatography test paper strip for quickly detecting perfluoro caprylic acid and preparation method thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0036] 2. Preparation of anti-perfluorooctanoic acid polyclonal antibody

[0037] Preparation of polyclonal antibody: New Zealand white rabbits were immunized with perfluorooctanoic acid carrier protein combined with antigen, the immunization dose was 1 mg / time, and multi-point subcutaneous injection was performed on the back. For the first immunization, mix the antigen with perfluorooctanoic acid carrier protein and the same amount of Freund's complete adjuvant (FCA), and fully emulsify; for booster immunization, mix the antigen with the carrier protein of perfluorooctanoic acid and the same amount of Freund's incomplete adjuvant (FIA), and fully emulsify , 3 weeks after the first immunization, 4-5 consecutive immunizations, each interval of 2-3 weeks, 10-15 days after the last immunization, the titer reached 10 by ELISA method 5 At the above time, blood was collected and serum was separated and collected. IgG antibodies were extracted by saturated ammonium sulfate salting-o...

Embodiment 1

[0042] Example 1: Spray 0.5 mg / ml of coating antigen and goat anti-rabbit IgG on the coating film as the test line (T) and control line (C) respectively, and dry in an oven at 37°C for 10 minutes. In the same way, a certain concentration of gold-labeled antibody was coated on the binding pad. The test strip is composed of a liner, on which a sample pad, a colloidal gold binding pad, a coating film and an absorbent pad are glued in sequence. Cut the affixed board into 3mm wide strips, and then put the test strips together with the desiccant into an aluminum foil bag and seal them for storage.

[0043] Detection operation method: insert the sample end of the perfluorooctanoic acid colloidal gold chromatography test strip into the sample solution to be tested, the insertion depth does not exceed the marked line, take out the test strip for about 10 to 20 seconds, place it horizontally, and observe and judge for about 15 minutes Test results.

[0044] Result judgment: If only on...

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Abstract

The invention relates to a colloidal gold chromatography test paper strip for quickly detecting perfluoro caprylic acid and a preparation method thereof, and belongs to the technical field of detection of biological immunization methods. The detecting test paper strip consists of a lining board, and a sample pad, a gold labeled combining pad, an enveloping film and a water absorbing pad, which are orderly connected on the lining board, wherein the gold labeled combining pad is glass fiber wool adsorbing gold-labeled antibodies of the perfluoro caprylic acid; and the enveloping film is provided with a linear invisible test line T line which is printed by carrier protein solution coupled by perfluoro caprylic acid, and a linear invisible contrast line C line which is printed by sheep anti-rabbit IgG solution, and the two lines are parallel. The test paper strip has strong specificity, high sensibility with the lowest detection limit of 5ppb, simplicity, convenience, quickness and strong timeliness, does not need any other reagents and instruments, can be operated in field, can determine a detection result in 15 minutes after being put into tested sample solution, has vivid, directviewing and accurate result display, saves cost, has wide applicable range and contributes to promotion.

Description

technical field [0001] The invention discloses a colloidal gold chromatography test strip for rapid detection of perfluorooctanoic acid and a preparation method thereof, which belong to the detection technical field of biological immune methods. Background technique [0002] Perfluorooctanoic acid, English name: Perfluorooctanoic acid (PFOA), CAS number 335-67-1, molecular formula C 8 HF 15 o 2 . Perfluorooctanoic acid and its salts (PFOA) is a commonly used fluorosurfactant, which has strong hydrophobicity and oleophobicity, and its chemical structure is far more stable than other surfactants. Due to its excellent heat resistance, low temperature resistance, self-lubricating property and chemical stability, etc., it is used in the fields of aerospace, electronics, chemistry and fire protection, and is used in life departments such as non-stick cookware, waterproof and breathable materials (such as waterproof clothing) ), leather, auto parts and microwave popcorn bags, e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/552
Inventor 胥传来李灼坤刘丽强徐丽广
Owner JIANGNAN UNIV
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