Modified glass fibers with monolayers of aminocalixarene derivatives and iminecalixarene derivatives, and oligo-dna modified glass fibers by fixing oligo-dna's to the monolayers, and the preparation m

A genotyping method, a technology for aminocalixarene, applied in the directions of biochemical equipment and methods, determination/inspection of microorganisms, analytical materials, etc.

Active Publication Date: 2009-10-28
BIOMETRIX TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0018] [18] In order to solve the problem of the traditional DNA chip by immobiliz

Method used

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  • Modified glass fibers with monolayers of aminocalixarene derivatives and iminecalixarene derivatives, and oligo-dna modified glass fibers by fixing oligo-dna's to the monolayers, and the preparation m
  • Modified glass fibers with monolayers of aminocalixarene derivatives and iminecalixarene derivatives, and oligo-dna modified glass fibers by fixing oligo-dna's to the monolayers, and the preparation m
  • Modified glass fibers with monolayers of aminocalixarene derivatives and iminecalixarene derivatives, and oligo-dna modified glass fibers by fixing oligo-dna's to the monolayers, and the preparation m

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Effect test

Embodiment 2

[0098] [94] A method of modifying an amine-modified glass fiber by using a solution (prepared by referring to a conventional method) in which 5,11,17,23-tetrabenzyliminoalkoxycalix[4]arene (TBICOCA) was dissolved.

[0099] [95]

[0100]

[0101] [96] TBICOCA

[0102] [97] 0.1~5.0mM 5,11,17,23-tetrabenzylimine alkoxycalix[4]arene (TBICOCA) in the derivative of formula 2 was dissolved in an organic solvent (such as CHCl 3 etc.) to prepare the solution. Such as image 3 As indicated, the glass fiber (for example, amine-based glass fiber substrate) to which the amine-based functional group is attached is immersed in the solution prepared in this way for 1-24 hours, and then the glass fiber is washed successively with chloroform, acetone and water, and then dried to A monolayer of the iminocalixarene of the present invention is formed. Another iminocalixarene derivative monolayer was prepared by referring to the same method.

[0103] [98]

[0104] [99] Example 3

[0105] ...

Embodiment 7

[0134] [123] Method for detecting streaks appearing on membrane of said modified glass fiber by measuring fluorescence after hybridization of probe DNAs to amplified gene

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Abstract

The present invention relates to a method of preparing a surface-modified glass fiber by bonding an aminocalixarene derivative or an iminecalixarene derivative on the surface of a glass fiber to form a monolayer, a method of preparing a glass fiber where oligo-DNAs are immobilized by immobilizing oligo- DNAs having consecutive guanine bases on the glass fiber prepared according to said method, and a method of preparing a rapid genotyping kit using the glass fiber where various oligo-DNAs are immobilized according to said method, thereby allowing genotyping of various genes.

Description

technical field [0001] [1] The present invention relates to a method for preparing a surface-modified glass fiber by bonding an aminocalixarene derivative or an iminocalixarene derivative to the surface of a glass fiber to form a monolayer, a A method of immobilizing oligomeric DNAs on glass fibers prepared according to the method to prepare glass fibers immobilized with oligomeric DNAs, and a rapid method using the glass fibers immobilized with various oligomeric DNAs prepared according to the method The preparation method of the genotyping kit supports genotyping of various genes by the aforementioned method. [0002] [2] Background technique [0003] [3] Since the completion of the Human Genome Map in 2000 in the Human Genome Project, which was carried out competitively by the Human Genome Research Group and Celera Genomics, research on gene function and mutation gene detection has been carried out worldwide, and the results of the research Oligo DNA chips were fabricat...

Claims

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Application Information

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IPC IPC(8): C12Q1/00
CPCG01N33/552
Inventor 金泰善宋锦秀金亨燮
Owner BIOMETRIX TECH
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